[8] Development and application of caged calcium
References (72)
Neuron
(1998)Curr. Opin. Neurobiol.
(1996)- et al.
Chem. Biol.
(1997) Tetrahedron Lett.
(1998)- et al.
Biophys. J.
(1996) - et al.
Cell Calcium
(1999) - et al.
Biophys. J.
(1994) - et al.
Neuron
(2001) - et al.
Neuron
(1996) - et al.
J. Biol. Chem.
(1996)
Trends Neurosci.
J. Physiol. (Paris)
Methods Cell Biol.
Neuron
Curr. Opin. Neurobiol.
J. Physiol.
J. Physiol.
Neuron
Nature (London)
Science
J. Neurobiol.
Neuron
Neuron
Biophys. J.
J. Neurosci.
J. Neurosci.
Biophys. J.
Neuron
Neuron
Neuron
Neuron
Neuron
Biophys. J.
J. Physiol.
J. Physiol.
Cell Calcium
Ber. Bunsenges. Phys. Chem.
J. Biol. Chem.
J. Cell Biol.
Nat. Neurosci
Science
Cited by (51)
Light-controlled gene expression in yeast using photocaged Cu<sup>2+</sup>
2017, Journal of BiotechnologyCitation Excerpt :Among the most successful applications of a commercially available caged compound is the photorelease of calcium ions from DMNP-EDTA (1-(2-nitro-4,5-dimethoxyphenyl)-N,N,N′,N′-tetrakis[(oxycarbonyl)methyl]-1,2-ethanediamine, DM-nitrophen) (Kaplan and Ellis-Davies, 1988). DMNP-EDTA-Ca has been used extensively in neurobiology, e.g., for the detailed investigation of synapse functionality (Ellis-Davies, 2003). Its success can be traced to the fact that it combines several features desirable for using caged compounds in biological environments, which include (i) no (or low) toxicity, (ii) stability in aqueous solutions, (iii) low premature release of the effector, biocompatible release wavelengths (λ > 300 nm), (iv) large extinction coefficients and high quantum yields, and (v) quantitative release of the effector molecule.
Optogenetic toolkit for precise control of calcium signaling
2017, Cell CalciumMeasuring the kinetics of calcium binding proteins with flash photolysis
2012, Biochimica et Biophysica Acta - General SubjectsCitation Excerpt :Of the most commonly used and commercially available caged-Ca2+ compounds DMn seems to be the best choice to release caged-Ca2+ when rapid and large increases in [Ca2+] are desired. First of all, DMn has the highest affinity for Ca2+ (Kd = 5 nM, the next best, NP-EGTA, has a Kd of 80 nM), and its PPs have the lowest affinity for Ca2+ after photolysis [60,61]. Consequently, about 95–99% of DMn is occupied with Ca2+ while the [Ca2+]rest is at 0.1–1 μM, and most of the bound Ca2+ will be released from the PPs.
The contribution of RCK domains to human BK channel allosteric activation
2012, Journal of Biological ChemistryRegulation of transmitter release by Ca<sup>2+</sup> and synaptotagmin: Insights from a large CNS synapse
2011, Trends in NeurosciencesPhotochemical reagents for the study of metalloproteins by flash photolysis
2011, Coordination Chemistry Reviews