Chapter 23 Quantification of Superoxide Production by Mouse Brain and Skeletal Muscle Mitochondria
Section snippets
Evaluation of Quantitative Methods for Detecting ROS Production
The reactive oxygen species: , H2O2, and the OH− radical are products of the single electron reduction of oxygen. The lifetime of these species is limited because of their high reactivity and the high capacity of radical scavenging reactions, thus to detect them in cells, tissue samples, and isolated organelles, very sensitive methods are required. Because ROS production strongly depends on oxygen concentration, in all experiments we used oxygen-saturated media to increase the sensitivity
The Application of Quantitative Methods for Measurement of ROS Production to Identify the Contribution of Individual Sites to the Superoxide Production of Isolated Brain and Skeletal Muscle Mitochondria
The particular impact of the potential sites relevant for the mitochondrial superoxide generation and its influence on mitochondrial metabolism is still a matter of dispute (Grivennikova et al., 2008, Komary et al., 2008, Kudin et al., 2008). Molecular oxygen is a triplet species that can accept only single electrons from potential donors (Naqui et al., 1986). This prevents oxygen (the midpoint potential of the couple is −0.33 V; Wood [1988]) from spontaneously oxidizing reduced
Acknowledgments
This study was supported by grants of the Deutsche Forschungsgemeinschaft (KU-911/15-1, the SBF TR3-A11, and TR3-D12) and the BMBF (01GZ0704) to W. S. K.
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