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2016, Cellular SignallingCitation Excerpt :PC12 cells that stably overexpress ERK5 shRNA or flag-ankrd1 were cultured in the presence of G418. Sympathetic neurons were dissociated from the superior cervical ganglia (SCG) of newborn rat pups as described [21,22]. Both sexes of rat pups were used without regard to sex.
Live-cell imaging of neurofilament transport in cultured neurons
2016, Methods in Cell BiologyCitation Excerpt :We focus here on our own current methods. Similar or alternative methods for culturing these neuronal cell types, which include detailed descriptions of the tissue dissection and culturing procedures, can be found elsewhere (Goslin, Hannelore, & Banker, 1998; Hawrot & Patterson, 1979; He & Baas, 2003; Higgins, Lein, Osterhout, & Johnson, 1991; Johnson & Argiro, 1983; Johnson, Iacovitti, Higgins, Bunge, & Burton, 1981; Kaech & Banker, 2006; Kleitman, Wood, & Bunge, 1998; Mahanthappa & Patterson, 1998). To establish and maintain primary neuronal cultures, we use fine dissecting instruments, a binocular dissecting microscope capable of 8–25x magnification, a horizontal laminar flow hood, and a tissue culture incubator with temperature and CO2 control as well as active or passive humidification.
Hierarchically structured nerve guidance channels based on poly-3-hydroxybutyrate enhance oriented axonal outgrowth
2014, Acta BiomaterialiaCitation Excerpt :To inhibit glial cell proliferation 0.24 μg ml−1 1-β-d-arabinofuranosylcytosine (Sigma) was added to the medium 24 h after seeding. SCG were cultured in Neurobasal A Medium (Life Technologies) supplemented with 1% N2-supplement (Gibco), Pen/Strep (200 U ml−1 and 200 mg ml−1), 0.7% B27 (Life Technologies), l-glutamine (2 mM), 10% FCS (Sigma) and NGF (100 ng ml−1, BD Bioscience) as previously described [47]. In order to allow Schwann cell growth and development, 1-β-d-arabinofuranosylcytosine was not added to SCG medium.
Involvement of CTP:phosphocholine cytidylyltransferase-β2 in axonal phosphatidylcholine synthesis and branching of neurons
2011, Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids