[20] Techniques in the tissue culture of rat sympathetic neurons
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Cited by (76)
Live-cell imaging of neurofilament transport in cultured neurons
2016, Methods in Cell BiologyCitation Excerpt :We focus here on our own current methods. Similar or alternative methods for culturing these neuronal cell types, which include detailed descriptions of the tissue dissection and culturing procedures, can be found elsewhere (Goslin, Hannelore, & Banker, 1998; Hawrot & Patterson, 1979; He & Baas, 2003; Higgins, Lein, Osterhout, & Johnson, 1991; Johnson & Argiro, 1983; Johnson, Iacovitti, Higgins, Bunge, & Burton, 1981; Kaech & Banker, 2006; Kleitman, Wood, & Bunge, 1998; Mahanthappa & Patterson, 1998). To establish and maintain primary neuronal cultures, we use fine dissecting instruments, a binocular dissecting microscope capable of 8–25x magnification, a horizontal laminar flow hood, and a tissue culture incubator with temperature and CO2 control as well as active or passive humidification.
Mitochondria-derived reactive oxygen species mediate caspase-dependent and -independent neuronal deaths
2014, Molecular and Cellular NeuroscienceCitation Excerpt :All other chemicals were purchased from Sigma (St. Louis, MO) unless otherwise noted. Cultures of sympathetic neurons were prepared from the superior cervical ganglia (SCG) of neonatal male and female C57BL/6 mice as described (Johnson and Argiro, 1983). ARRIVE guidelines were followed.
Bax affects production of reactive oxygen by the mitochondria of non-apoptotic neurons
2007, Experimental NeurologyCell cycle events distinguish sensory neuronal death from motoneuron death as a result of trophic factor deprivation
2003, Molecular and Cellular NeuroscienceJNK-independent activation of c-Jun during neuronal apoptosis induced by multiple DNA-damaging agents
2003, Journal of Biological ChemistryCitation Excerpt :Alsterpaullone was obtained from Calbiochem. Neuronal Culture—Primary sympathetic neuronal cultures were prepared from P0 to P1 rat superior cervical ganglia by using methods described previously (28, 29). Briefly, superior cervical ganglia were dissected from newborn animals and incubated for 30 min each with 1 mg/ml collagenase and 2.5 mg/ml trypsin at 37 °C.