Elsevier

Methods in Enzymology

Volume 103, 1983, Pages 334-347
Methods in Enzymology

[20] Techniques in the tissue culture of rat sympathetic neurons

https://doi.org/10.1016/S0076-6879(83)03022-0Get rights and content

Publisher Summary

This chapter describes the techniques for the tissue culture of rat sympathetic neurons. The superior cervical ganglion (SCG), rather than the sympathetic chain ganglia, has been used as the neuronal source. The SCG has been well studied in vivo and, because of its size and accessibility, it is easily dissected. The dissection of the SCG from postnatal rats differs only in some details from that described for the embryonic ganglion. For all ages ether anesthesia can be used and the dissection done after appropriate sterile preparation of the neck. Three sets of sterile instruments for the dissection of the skin, glands, muscles, and SCG helps in the sterile removal of the ganglion. A faster method avoiding the possible membrane effects of ether anesthesia uses decapitation.

References (36)

  • C.-P. Ko et al.

    Brain Res.

    (1976)
  • M.I. Olson et al.

    Brain Res.

    (1973)
  • D. Higgins et al.

    Neuroscience

    (1982)
  • L. Iacovitti et al.

    Neuroscience

    (1982)
  • E. Hawrot

    Dev. Biol.

    (1980)
  • P.M. Wood

    Brain Res.

    (1976)
  • F.A. Mithen et al.

    Neurosci. Lett.

    (1980)
  • B.S. Scott

    Exp. Neurol.

    (1971)
  • V. Argiro et al.

    J. Neurosci.

    (1982)
  • D. Higgins et al.

    J. Neurosci.

    (1981)
  • M. Johnson et al.

    Nature (London)

    (1976)
  • L. Iacovitti et al.

    J. Neurosci.

    (1981)
  • V. Argiro et al.

    J. Cell. Biol.

    (1981)
  • M.B. Bunge

    J. Cell Biol.

    (1973)
  • R.P. Rees et al.

    J. Cell Biol.

    (1976)
  • P.H. Patterson et al.
  • L.S. McLennan et al.

    Nature (London)

    (1980)
  • P.A. Walicke et al.
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