[37] Immunoelectron microscopy using thin, frozen sections: Application to studies of the intracellular transport of Semliki Forest virus spike glycoproteins
References (47)
- et al.
J. Mol. Biol.
(1978) - et al.
Immunochemistry
(1977) J. Ultrastruct. Res.
(1978)- et al.
J. Mol. Biol.
(1973) - et al.
Biochim. Biophys. Acta
(1976) - et al.
Virology
(1969) - et al.
Lancet
(1966) - et al.
J. Mol. Biol.
(1981) - et al.
J. Mol. Biol.
(1981) - et al.
J. Gen. Virol.
(1980)
Nature (London)
Nature (London)
J. Gen. Virol.
J. Histochem. Cytochem.
J. Cell Biol.
Immunofluorescence and Related Staining Techniques
Histochem. J.
Immunochemistry
J. Histochem. Cytochem.
J. Cell Biol.
J. Cell Biol
J. Cell Biol.
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2015, Journal of Structural BiologyCitation Excerpt :Nevertheless, correlating light and electron micrographs has proven to be challenging due to the different constraints imposed by the type of microscopy performed. To ensure easy transfer of a precisely located biological region from the light microscope to the electron microscope, we developed a complete process based on the cryo-sectioning method for sample preparation established by Tokuyasu (Griffiths et al., 1983; Slot and Geuze, 2007; Tokuyasu, 1973). This method was chosen for two main reasons:
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2008, Methods in Cell BiologyCitation Excerpt :Chemically fixed biological specimens have also been processed for immunocytochemistry by rapid dehydration at ambient temperature and resin embedded using heat polymerization (Gocht, 1992; Osamura et al., 2000; Waller et al., 1998). A popular, but specialized method for preparing biological material for immunolabelling is the Tokuyasu cryosectioning technique (Griffiths et al., 1983; Tokuyasu, 1980). It is often the preferred immunolabelling method because it is the only post-embedding immunolabelling approach that does not require dehydration by polar solvents before application of affinity markers.