Ich-1, an Ice/ced-3-related gene, encodes both positive and negative regulators of programmed cell death

doi:10.1016/S0092-8674(94)90422-7

Cell

Volume 78, Issue 5, 9 September 1994, Pages 739-750

https://doi.org/10.1016/S0092-8674(94)90422-7 Get rights and content

Summary

We report here the isolation and characterization of Ich-1, a gene related to the C. elegans cell death gene ced-3 and the mammalian homolog of ced-3, interleukin-1β-converting enzyme (ICE). Alternative splicing results in two distinct Ich-1 mRNA species. One mRNA species encodes a protein product of 435 amino acids (ICH-1_L) that is homologous to both the P20 and P10 subunits of ICE (27% identity) and the entire CED-3 protein (28% identity). The other mRNA encodes a 312 amino acid truncated version of ICH-1_L protein (ICH-1_s). Overexpression of Ich_L induces programmed cell death, suggesting that Ich-1 is also a mammalian programmed cell death gene. More interestingly, overexpression of the Ich-1_s suppresses Rat-1 cell death induced by serum deprivation. These observations suggest that Ich-1 plays an important role in both positive and negative regulation of programmed cell death in vertebrate animals.

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Summary

References (19)

bcl-x, a bcl-2-related gene that functions as a dominant regulator of apoptotic cell death

Cell

Interleukin-1 and interleukin-1 antagonism

Blood

Identification of a set of genes with developmentally down-regulated expression in the mouse brain

Biochem. Biophys. Res. Commun.

Induction of apoptosis in fibroblasts by IL-1β-converting enzyme, a mammalian homolog of the C. elegans cell death gene ced-3

Cell

Expressed cerebellar-type inositol 1,4,5-triphosphate receptor, P₄₀₀, has calcium release activity in a fibroblast L cell line

Neuron

Post-embryonic cell lineages of the nematode Caenorhabditis elegans

Dev. Biol.

The embryonic cell lineage of the nematode Caenorhabditis elegans

Dev. Biol.

An evolutionary perspective on apoptosis

Cell

Genetic mosaic analysis of ced-3 and ced-4, two genes that control programmed cell death in the nematode C. elegans

Dev. Biol.

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ArticleIch-1, an Ice/ced-3-related gene, encodes both positive and negative regulators of programmed cell death

Summary

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Biochem. Biophys. Res. Commun.

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Dev. Biol.

Dev. Biol.

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Article
Ich-1, an Ice/ced-3-related gene, encodes both positive and negative regulators of programmed cell death