Localization of 5-HT4 receptor mRNA in rat brain by in situ hybridization histochemistry

doi:10.1016/S0169-328X(96)00248-3

Molecular Brain Research

Volume 43, Issues 1–2, 31 December 1996, Pages 356-360

https://doi.org/10.1016/S0169-328X(96)00248-3 Get rights and content

Abstract

Oligonucleotide probes that recognize two cloned splice variants (5-HT_4S and 5-HT_4L) of 5-HT₄ receptors were used to study by in situ hybridization the localization in rat brain of mRNA encoding these receptors. A probe common to both variants reveals high levels of transcripts in olfactory tubercle, some components of the basal ganglia (caudate putamen, ventral striatum), medial habenula and hippocampal formation. Similar patterns of distribution are obtained with probes that recognize each splice variant individually, suggesting that no dramatic differences exist in their respective regional distribution. Comparison of mRNA distribution with receptor distribution as visualized with [¹²⁵I]SB 207710 indicates that 5-HT₄ receptors are localized both somatodendritically in e.g. caudate putamen and on axon terminals in e.g. substantia nigra and globus pallidus.

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Acknowledgements

This work was supported by Grant 94/0864 from the Fondo de Investigación Sanitaria (FIS). Support from the CIRIT (Generalitat de Catalunya) to the Department of Neurochemistry (IIBB/CSIC) as Grup de Recerca de Qualitat (1995 SGR 00445) is acknowledged.

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Citation Excerpt :
The distribution of the mRNAs encoding the different C-terminal splice variants of 5-HT4 receptors has been studied by in situ hybridization or by RT-PCR. In the rat brain, in situ hybridization (Vilaró et al., 1996) or RT-PCR (Claeysen, Sebben, Journot, Bockaert, & Dumuis, 1996) showed that 5-HT4a and 5-HT4b variants present a widespread and very similar regional distribution. Another RT-PCR study of the distribution of 4(a), 4(b), and 4(e) C-terminal variants in the rat has shown that r5-HT4(b) mRNA presents the widest distribution, being strongly detected in all the regions examined (olfactory tubercle, striatum, hippocampus, parietal cortex, substantia nigra, and inferior colliculus).
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¹: Permanent address: Research Institute, Laboratorios Almirall, 08024 Barcelona, Spain.

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Short communicationLocalization of 5-HT4 receptor mRNA in rat brain by in situ hybridization histochemistry

Abstract

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Acknowledgements

Short communication
Localization of 5-HT₄ receptor mRNA in rat brain by in situ hybridization histochemistry