Quantification of tau phosphorylated at threonine 181 in human cerebrospinal fluid: a sandwich ELISA with a synthetic phosphopeptide for standardization
Section snippets
Acknowledgements
This work was supported by a grant from the ‘Vlaams Instituut voor de bevordering van het Wetenschappelijk Technologisch onderzoek in de industrie’ (Grant 990220) and the Medical Research Council, Sweden, Grant No. 12103 and 11560. Fred Shapiro is thanked for critical review and editing of the manuscript and Bob DeLeys for the initial experiments on the epitope mapping.
References (17)
- et al.
The structural basis of monoclonal antibody Alz50’s selectivity for Alzheimer's disease pathology
J. Biol. Chem.
(1996) - et al.
Tau proteins of Alzheimer paired helical filaments: abnormal phosphorylation of all six brain isoforms
Neuron
(1992) - et al.
Multiple isoforms of human microtubule-associated protein tau: sequences and localization in neurofibrillary tangles of Alzheimer's disease
Neuron
(1989) - et al.
Phosphorylated tau in human cerebrospinal fluid is a diagnostic marker for Alzheimer's disease
Neurosci. Lett.
(1999) - et al.
Epitopes that span the tau molecule are shared with paired helical filaments
Neuron
(1988) - et al.
Biopsy-derived adult human brain tau is phosphorylated at many of the same sites as Alzheimer's disease paired helical filament tau
Neuron
(1994) - et al.
In vivo phosphorylation sites in fetal and adult rat tau
J. Biol. Chem.
(1993) - et al.
Tau protein in cerebrospinal fluid: a biochemical marker for axonal degeneration in Alzheimer disease?
Mol. Chem. Neuropathol.
(1995)
There are more references available in the full text version of this article.
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