Calbindin and Fos within the suprachiasmatic nucleus and the adjacent hypothalamus of Arvicanthis niloticus and Rattus norvegicus
Section snippets
Animals
We used adult (>60 days) A. niloticus bred in the laboratory from a stock descended from 29 individuals originally trapped in Kenya in 1993.15 In addition, we used adult male Rattus (Charles River Laboratories, Cambridge, MA, USA). All animals were kept in a 12:12 LD cycle, singly housed in Plexiglas cages (38×34×16 cm) and provided with water and food (Harlan 8640 Teklad, Madison, WI, USA) ad libitum. A red light (<5 lux) remained on constantly for the purposes of animal care. All experiments
Suprachiasmatic nucleus of Arvicanthis niloticus
CALB-immunoreactive expression within a section through the central SCN of a representative Arvicanthis is depicted in Fig. 2A. There was a very distinct pattern of CALB-immunoreactivity within the SCN; the most centrally located CALB-immunoreactive neurons were more densely concentrated and darkly stained than the CALB-immunoreactive neurons in the more peripheral regions of the nucleus (Fig. 2A). We saw no obvious differences between males and females with respect to the distribution of
Discussion
The distribution of CALB-immunoreactive cells within the SCN of Arvicanthis was similar in some respects to that described in M. auratus. Specifically, in both species the central portion of the SCN contained the highest concentration of darkly stained CALB-immunoreactive cells.46 These two species differed slightly in that more lightly stained CALB-immunoreactive cells were found outside the central portion of the SCN in Arvicanthis (2., 3.) than in M. auratus.46 Far more cells positive for
Acknowledgements
The authors would like to thank Teresa McElhinny, Betty Gubik, Colleen Novak, and Sandra Rose for their valuable assistance and advice. The research described in this paper was supported by NIMH RO1-MH053433 to L.S. and NSF IBN 9514374 to A.A.N.
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2007, Brain ResearchCitation Excerpt :Interestingly, the pattern of distribution observed in the grass rat LSPV was similar to the one observed in the SCN of this species, suggesting that these inputs could be directed to a specific population of neurons, which resides in the caudal SCN–LSPV area. As in the caudal SCN, neurons in the caudal LSPV express the protein CalB (Mahoney et al., 2000). Nothing is known about the role that CalB neurons play in the LSPV of grass rats, but these neurons are likely to be involved in circadian rhythmicity since CalB expression changes as a function of time in the LSPV of grass rats and the rhythm persists in constant darkness (Schwartz et al., 2004).
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