Prolonged effects of polyriboinosinic:polyribocytidylic acid on spontaneous running wheel activity and brain interferon-α mRNA in rats: a model for immunologically induced fatigue
Section snippets
Animals
Male Wistar rats weighing 250–300 g were housed individually in plastic cages (25×42×18 cm) at a temperature of 23–25 °C with 12-h light/dark cycle (light on at 08:00 h) and had free access to a laboratory chow and water. Each cage contains 30-cm-diameter running wheel (SW-30; Melquest Inc., Toyama, Japan) and rats can enter the wheel any time. The number of wheel turns per minute was counted using analog/digital interface (CIF-32) and computer software from Melquest Inc. Experiments started
Effects of poly I:C on running wheel activity
As shown in Fig. 1, an actogram of a rat given i.p. injection of poly I:C (3 mg/kg) demonstrated a decrease in running wheel activity after the injection performed on day 1, lasting for at least 1 week. To further analyze the pattern of running activity, histograms for the number of wheel turns in 1-min bin (w.t./min) were made from data on days (−2), 1, and 7. As shown in Fig. 2A, upper, there were two peaks of 6 and one w.t./min before poly I:C on day (−2). The raw data indicated that the
Discussion
Voluntary running in the wheel is a highly motivated activity in rats, which run about 800–1800 w.t./day in normal condition. The present results indicated that i.p. injection of poly I:C (3 mg/kg) in rats induced a decrease in daily amount of spontaneous running wheel activity to about 40–60% of the preinjection level until day 9, while circadian rhythm of the activity was maintained. The running wheel activity gradually returned to the baseline level by day 14. mRNA for IFN-α increased in the
Acknowledgements
This work was supported by Grant-in Aid for Scientific Research (14657018 and 12470011 (B) to T.K.) and Special Coordination Funds of the Ministry of Education, Culture, Sports, Science and Technology, the Japanese Government.
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