Parvalbumin-immunoreactive neurons in the hippocampal formation of Alzheimer's diseased brain
Section snippets
Tissue preparation
Eight human brains with no history of neurological or neuropsychological illness (mean age 64.2±6.2 years, range 28–81 years) and 12 brains of clinically diagnosed and pathologically confirmed25, 29AD (mean age 81.2±2.0 years, range 69–89 years) were obtained at autopsy (mean post-mortem delay: aged controls, 4.75±0.8 h; AD, 4.5±0.4 h; Table 1). The brains were sliced transversely into 1-cm slabs, immersion fixed for 24 h in cold (4°C) 0.1 M phosphate-buffered–4% paraformaldehyde (pH 7.4) and then
Results
We followed the cytoarchitectural subdivisions of the hippocampal formation defined by Ramon y Cajal[36]and Lorente de No[27]as previously reported.[6]In brief, the hippocampal formation consists of the dentate gyrus along the medial edge of the temporal lobe, with subfields of the hippocampus proper emanating from the hilus of the dentate gyrus (CA4), progressing through subfields CA3, CA2 and CA1 to the subiculum and adjacent presubiculum (Fig. 1a).
Parvalbumin immunostaining was observed
Discussion
This report demonstrates the selective vulnerability of parvalbumin-immunoreactive neurons within hippocampal subfields of AD brain. When interpreting the results in the context of neural systems, the present study is consistent with previous reports of both resistance and vulnerability of the parvalbumin neuronal population in AD. These findings support and expand previous neuropathological data on the predilection of certain hippocampal subfields to degeneration in AD.6, 21
Acknowledgements
We thank L. Sue, G. Binskin and J. Schaller for excellent technical assistance. We are indebted to the families whose donation of brain tissue is the basis of this report. Supported by NS 26146, AG 10688, AG 11482, AG 09466 and AG 10161.
References (45)
- et al.
Loss of parvalbumin immunoreactive neurons from cortex in Alzheimer-type dementia
Brain Res.
(1987) Calbindin D-28k and parvalbumin in the rat nervous system
Neuroscience
(1990)- et al.
Decreased somatostatin immunoreactivity but not neuropeptide Y immunoreactivity in cerebral cortex in senile dementia of Alzheimer type
Neurosci. Lett.
(1986) - et al.
Parvalbumin immunoreactive neurons in normal human temporal neocortex and in patients with Alzheimer's disease
J. neurol. Sci.
(1991) - et al.
Parvalbumin-immunoreactive dystrophic neurites and aberrant sprouts in the cerebral cortex of patients with Alzheimer's disease
Neurosci. Lett.
(1993) - et al.
Chandelier cell axons identified by parvalbumin-immunoreactivity in the normal human temporal cortex and in Alzheimer's disease
Neuroscience
(1993) - et al.
Subpopulations of somatostatin-28 immunoreactive neurons display different vulnerability in senile dementia of the Alzheimer type
Brain Res.
(1989) - et al.
Immunoreactive parvalbumin concentration in parahippocampal gyrus decreases in patients with Alzheimer's disease
J. neurol. Sci.
(1992) - et al.
Demonstration of neurofibrillary tangles in parvalbumin-immunoreactive interneurones in the cerebral cortex of Alzheimer-type dementia brain
Neurosci. Lett.
(1991) - et al.
Immunohistochemical patterns of selective cellular vulnerability in human cerebral ischemia
J. neurol. Sci.
(1993)
Distribution of immunoreactive cholecystokinin in the human hippocampus
Peptides
Most somatostatin immunoreactive neurons in the rat fascia dentata do not contain the calcium-binding protein parvalbumin
Brain Res.
GABAergic hippocampal neurons resistant to ischemia-induced neuronal death contain the Ca++-binding protein parvalbumin
Neurosci. Lett.
Differential sensitivity of calbindin and parvalbumin immunoreactive cells in the striatum to excitotoxins
Brain Res.
Use of cryoprotectant to maintain long-term peptide immunoreactivity and tissue morphology
Peptide
Transmitter systems in the primate dentate gyrus
Hum. Neurobiol.
Parvalbumin in human brain
J. Neurochem.
Parvalbumin-immunoreactive structures in the hippocampus of the human adult
Cell Tissue Res.
Amygdaloid pathology in Alzheimer's disease: qualitative and quantitative analysis
Dementia
Alz50 immunoreactive neuropil differentiates hippocampal complex subfields in Alzheimer's disease
J. comp. Neurol.
Parvalbumin in most γ-aminobutyric acid-containing neurons of the rat cerebral cortex
Science
Somatostatin immunoreactive neurons in the human hippocampus and cortex shown by immunogold/silver intensification on vibratome sections: coexistence with neuropeptide Y neuronism, and effects in Alzheimer-type dementia
J. comp. Neurol.
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