Area- and lamina-specific organization of a neuronal subpopulation defined by expression of latexin in the rat cerebral cortex
Section snippets
Animals
Wistar male rats (Sankyo; Shizuoka, Japan) aged 45–55 days were used.
Timm staining
To facilitate the identification of cortical areas, sections were stained by the Timm method,[16]with certain modifications compatible with simultaneous detection of latexin immunoreactivity. Rats (n=3) were deeply anaesthetized with pentobarbital (50 mg/kg) and perfused sequentially, first with 100 ml of 0.1% sodium sulphide in 0.15 M sodium phosphate buffer (pH 7.4), then with 250 ml of 0.5% glutaraldehyde in the same buffer,
Density and laminar distribution of latexin-immunoreactive neurons
As described in our previous reports,2, 3latexin-immunoreactive neurons were found in the lateral part of the neocortex and in the adjacent ventral periallocortex. With the modified fixation protocol, the pattern of Timm staining in the cerebral cortex was similar to that reported by Zilles et al.[84]and by Pérez-Clausell.[59]We found that the density of latexin-immunoreactive neurons in the cerebral cortex co-varies with the subdivision of the lateral cortex into areas defined by the Nissl and
Density and laminar distribution of latexin-immunoreactive neurons and their correlation with cortical area organization
We have established that latexin-positive neurons are abundant in the infragranular layers of the lateral cortical fields, but are very rare or completely absent from dorsal and medial cortical fields. Furthermore, we have demonstrated that the density and laminar distributions of latexin-positive neurons co-vary in relation to cytoarchitectonic area boundaries as defined by Zilles and colleagues,82, 83with the exception of Oc2L.
Interestingly, latexin-positive neurons were dense throughout the
Acknowledgements
The authors wish to thank Drs Edward G. Jones, Heinz Künzle, Toshio Terashima and Keiko Takiguchi-Hayashi for their comments on the manuscript. This work was supported in part by a research grant from the Human Frontier Science Program (RG-11/96 B).
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2009, NeuroscienceCitation Excerpt :In the cat primary somatosensory cortex (areas 1+2+3b), latexin+ neurons were distributed moderately densely in the laterodorsal part and sparsely in the most dorsal part, and were absent in the medial part. In the rat SI area, they were distributed in the most lateral part but were absent in the laterodorsal and dorsal parts (Arimatsu et al., 1999). In both species, latexin+ neurons were linked with the cutaneous sensory input from the head and face regions, rather than with other body surfaces (Felleman et al., 1983; Chapin and Lin, 1984).