Biochemical and Biophysical Research Communications
Proteomic analysis of nicotine-associated protein expression in the striatum of repeated nicotine-treated rats
Section snippets
Materials and methods
Animals and nicotine treatment. Male Sprague–Dawley rats weighing 250–280 g at the start of experiments were used throughout. Prior to the experimental manipulation, the animals were given a period of 1 week to adjust to the new environment. Animals were cared for and handled in accordance with the National Institutes of Health Guidelines for the Care and Use of Laboratory Animals. Animals were divided randomly into nicotine-treated (NIC, n = 10) and control (CONT, n = 10) groups. For nicotine
Results and discussion
Tobacco smoking still remains a major health problem worldwide. Nicotine is a principal component of tobacco, eliciting addictive behavior in human [1], [5], [6]. Intermittent nicotine administration brings about the behavioral sensitization, which is defined as an increased behavioral and/or neurochemical response [8]. Locomotor activation and reinforcement may be relevant to the stimulatory effects of nicotine on the mesolimbic dopaminergic system [18]. The striatum and nucleus accumbens are
Acknowledgments
The authors thank Prof. Kang-Duk Choi at Hankyong National University for the technical support and the helpful discussion. This study was supported by the Brain Korea 21 Project from the Ministry of Education, Republic of Korea.
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