Biochemical and Biophysical Research Communications
Extensive modulation of a set of microRNAs in primary glioblastoma
Section snippets
Materials and methods
RNA isolation from tissue samples and cell lines. Tissue samples were obtained after informed consent from adult patients diagnosed with glioblastoma de novo, one astrocytoma grade II, and one oligoastrocytoma grade III, freshly resected during surgery and immediately frozen in liquid nitrogen for subsequent total RNA extraction. RNA was isolated from tissues and cell lines using Trizol reagent (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions. Human cell lines used in
Results and discussion
To investigate whether miRNAs are differentially expressed in glioblastoma versus normal brain tissue, we performed a microarray analysis using a microarray chip able to examine global expression levels of 245 miRNAs [16]; the chip also enabled to distinguish between the mature and precursor form (pre-miRNA) of 76 microRNA sequences.
By this method, we analyzed tissue samples from nine primary glioblastoma patients (five female and four male). In order to yield a very specific, case by case
Acknowledgments
This study was funded in part by grants from the Italian Ministry of Instruction, University and Scientific Research (MIUR), FIRB 2001 and CNR-MIUR “Progetto Oncologia,” to M.G.F. and to G.M., by Associazione Italiana per la Ricerca sul Cancro (AIRC), by Ministero dell’Istruzione, dell’Università e della Ricerca by Ministero della Salute Italiano and by Progetto CAN2005—Comitato dei Sostenitori to M.N., by Program Project Grant P01CA76259, P01CA81534, and CA083698 to C.M.C from the National
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These two authors contributed equally to this work.