Original articleAssociation Between Polymorphisms in the Promoter Region of the Sialyltransferase 8B (SIAT8B) Gene and Schizophrenia
Section snippets
Subjects
For the case-control study, we recruited 188 unrelated schizophrenics, of whom 98 were male (mean age 54.2 ± 7.9) and 90 female (mean age 54.8 ± 12.7). All patients were diagnosed according to the Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition (DSM-IV) (American Psychiatric Association 1994), to give best-estimate lifetime diagnosis, with consensus from at least two experienced psychiatrists. All available medical records and family informant reports were also taken into
Detection of the SIAT8B and SIAT8D Gene Polymorphisms
The genomic layout of the human SIAT8B and SIAT8D genes, which span 70.3 kb on 15q26 and 96.1 kb on 5q21, respectively, are shown in Figure 1. The transcription start sites of both genes have been confirmed experimentally (Scheidegger et al 1995; http://dbtss.hgc.jp/). The SIAT8B gene is composed from 6 exons, and SIAT8D of 5 exons. Based on this structural information, we performed mutation screening of the genes, inspecting all exons, splice boundaries and the 5′ upstream region using 20
Discussion
While both SIAT8B and SIAT8D regulate the annexation of negatively charged oligosaccharides onto PSA, this study suggests that only SIAT8B may play a role in conferring susceptibility to Japanese schizophrenia. Despite the fact that polysaccharides are abundantly expressed in the brain and their crucial role in development and synaptic plasticity in the central nervous system, there have been no investigations of association between polysaccharide-synthesizing genes and schizophrenia. To our
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