Original ContributionResveratrol interacts with estrogen receptor-β to inhibit cell replicative growth and enhance stress resistance by upregulating mitochondrial superoxide dismutase
Section snippets
Materials
Modified Eagle medium with Earl salts, l-glutamine, and sodium bicarbonate; Dulbecco's modified Eagle medium with high glucose, l-glutamine, and sodium bicarbonate; and Dulbecco's modified Eagle medium:nutrient mixture F-12 were obtained from Sigma–Aldrich (St. Louis, MO, USA). Penicillin/streptomycin, nonessential amino acids, and fetal bovine serum were obtained from Hyclone (Logan, UT, USA). Resveratrol was obtained from A.G. Scientific (San Diego, CA, USA). Sirtinol and diarylpropionitrile
Results
Many of RES's reported effects are consistent with an increase in cellular stress resistance [1]. To investigate the long-term effects of RES on stress resistance we incubated three mammalian cell lines with RES for 48 h, and removed it before exposure to the oxidizing agents hydrogen peroxide and paraquat and the methylating agent methylmethanesulfonate (MMS). Pretreatment with RES significantly increased resistance to hydrogen peroxide and paraquat in all three cell lines. Similarly, a
Discussion
Two of RES's most exciting effects in the context of human health are its abilities to protect cells against stress-induced death and to slow rates of proliferative cell growth. Here we have shown that both of these effects are concurrent with, and dependent upon, the ability of RES to increase protein levels and activity of MnSOD. This observation is particularly interesting given the importance of MnSOD. In many cell types, mitochondria are the primary producer of intracellular reactive
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2021, Nutraceuticals: Efficacy, Safety and ToxicityPrimary hippocampal estrogenic dysfunction induces synaptic proteins alteration and neuronal cell death after single and repeated paraquat exposure
2020, Food and Chemical ToxicologyCitation Excerpt :Finally, E2 co-treatment with PQ partially reversed the ROS generation and the lipid and protein oxidation. Although there are no studies of PQ effects on estrogenic action, previous studies reported that E2 treatment reverts cell loss and oxidative stress seen after PQ administration (Robb and Stuart, 2011; Si et al., 2001). This supports our hypothesis of a possible anti-estrogenic effect of PQ on oxidative stress generation and cell death.
Selenium deficiency impaired structural integrity of the head kidney, spleen and skin in young grass carp (Ctenopharyngodon idella)
2018, Fish and Shellfish ImmunologyCitation Excerpt :However, so far, no studies have investigated the impacts of selenium deficiency on antioxidant and apoptosis as well as the possible signalling pathways in fish immune organs. In human, studies demonstrated that selenium supplement elevated the expression of estrogen receptor β (ER β) in MDA-MB231 cells [19], and increasing ER β level could up-regulate the mRNA level of MnSOD in MRC5 cells [20]. Additionally, studies on human also discovered that selenium deficiency up-regulated the mRNA level of Vascular Endothelial Growth Factor (VEGF) in PC3 cells [21], and upregulation of VEGF expression could activate the Nrf2 signalling pathway in BeWo cells [22].
Effects of exercise training and resveratrol on vascular health in aging
2016, Free Radical Biology and MedicineCitation Excerpt :Resveratrol has been shown to activate the SIRT1/AMPK/LKB-1 pathway [88] although there is also evidence for that resveratrol can activate the AMPK/LKB-1 pathway independently of SIRT1 [89]. Finally, resveratrol binds to estrogen receptors, albeit with a lower affinity than estrogen [90], and induces expression of a number of genes, including antioxidant genes [91]. Low concentrations of resveratrol can also activate eNOS through estrogen receptor-alpha on the endothelium [92].