Elsevier

Neuroscience Letters

Volume 475, Issue 3, 21 May 2010, Pages 121-123
Neuroscience Letters

Effect of in vivo striatal perfusion of lipopolysaccharide on dopamine metabolites

https://doi.org/10.1016/j.neulet.2010.03.050Get rights and content

Abstract

We have used the microdialysis technique to perfuse different concentrations of LPS in the rat's striatum 24 h after the implantation of a microdialysis probe. Dopamine metabolites in the dialysate obtained from the rat brain were measured by HPLC using electrochemical detection. Results show that intrastriatal perfusion of different concentrations of LPS produced a dose-dependent decrease in the extracellular DOPAC output, with no effect on the extracellular HVA output. Since DOPAC levels reflect the intraneuronal metabolism of dopamine while and HVA levels reflect the extraneuronal one, we suggest that in vivo intrastriatal LPS perfusion especially affects the level of newly synthesized dopamine or intraneuronal dopamine catabolism.

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Acknowledgements

This study was supported by Grant SAF2007-62425 from Spanish Government. R.M. thanks the Spanish Ministerio de Educación y Ciencia for an FPI fellowship.

References (25)

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