Pharmacological characterization of GABAB receptor subtypes assembled with auxiliary KCTD subunits
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Keywords
GABA-B
G-protein-coupled receptor
GS93783
Baclofen
Kir3
KCTD
Abbreviations
3KO
Kctd8/12/16−/− triple knockout mice
A1
adenosine receptor 1
ANOVA
analysis of variance
BRET
bioluminescence resonance energy transfer
CHO cells
Chinese hamster ovary K1 cells
CHO-GABAB
CHO cells stably expressing GABAB(1b,2) receptors
CGP7930
2,6-Di-tert-butyl-4-(3-hydroxy-2,2-dimethyl-propyl)-phenol
ΔBRET
changes in BRET
EC50
half maximal effective concentration
EGFP
enhanced green fluorescent protein
Emax
maximum stimulatory effect
GABABR
GABAB receptor
GDP
guanosine 5'-diphosphate
GPCR
G-protein-coupled receptor
GS39783
N,N'-Dicyclopentyl-2-methylsulfanyl-5-nitro-pyrimidine-4,6-diamine
[35S]GTPγS
[35S]guanosine 5′-O-(3-thio)triphosphate
HEK293T cells
Human Embryonic Kidney 293T cells
HIV-1
human immunodeficiency virus-1
IC50
half maximal inhibitory concentration
KCTD
Potassium Channel Tetramerisation Domain
KH buffer
Krebs-Henseleit buffer
Kir
K+ inwardly rectifying
mBU
milli BRET units
PAM
positive allosteric modulator
Rluc
Renilla reniformis luciferase
Cited by (0)
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These authors contributed equally to this work.
Copyright © 2014 The Authors. Published by Elsevier Ltd.