HuC/D confocal imaging points to olfactory migratory cells as the first cell population that expresses a post-mitotic neuronal phenotype in the chick embryo
Section snippets
Experimental procedures
Fertilised chick eggs were kept in a “Covatutto 40” incubator (Novital, Varese, Italy) at 38 °C and 80% relative humidity. Embryos were withdrawn at various developmental stages according to Hamburger and Hamilton (1992): 16 HH (about 51–56 h of incubation), 18 HH (about 65–69 h of incubation), 21 HH (about 3.5 days of incubation), and 30 HH (about 6.5 days of incubation). Approval for this study was obtained from the Animal Care and Ethics Committee of the University of Turin.
Embryos were
Results
Results of the HuC/D immunolabelling are shown in Fig. 1. At stage 16 HH, about 51–56 h of incubation, a cluster of a-HuC/D immuno-positive cells projecting from the olfactory pit to the telencephalic vesicle was detected (Fig. 1a). Only occasional labeled cells were detected in the context of the olfactory neuroepithelium that is just beginning to hollow and to form a pit. High resolution confocal imaging (Fig. 1e) demonstrated the prevailing cytoplasmic location of HuC/D antigens.
At stage 18
Discussion
The main finding of the present study is the identification and description of a nerve cell population that first, and massively, shows immuno-positivity for the anti-HuC/D antibody in comparison to other regions of the developing chick nervous system where immmuno-labeling by the antibody directed against these two RNA-binding proteins appears later (Wakamatsu and Weston, 1997). In the chick embryo, the early stages of olfactory neuroepithelium differentiation are accompanied by migration of
Acknowledgements
This work was supported by grants from the Italian MURST/MIUR.
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