Sensory systemAfferent regulation of oxidative stress in the chick cochlear nucleus
Section snippets
Subjects
All subjects were Ross×Ross chickens that were hatched and reared at Florida State University. The procedures used in these experiments were approved by the Animal Care and Use Committee at The Florida State University and conform to the guidelines set forth by the National Institutes of Health. All efforts were made to minimize the number of animals used and their potential suffering. Subjects were 7–10 days posthatch for the experiments using immunocytochemistry. This age was chosen because
Deafferentation-induced elevation in levels of ROS
Unilateral cochlea removal resulted in a transient rise in levels of ROS in the NM neurons. An example of this effect is shown in Fig. 1. Approximately 6–8 h following cochlea removal, deafferented NM neurons exhibit elevated levels of ROS. Opposite sides of the same tissue section were compared for analysis and these objective analyses of staining density confirmed the visual impressions. A two-way mixed analysis of variance (ANOVA) was performed on the gray scale density measurements using
Discussion
The chick brain stem provides a valuable model to explore a possible novel pathway in which ROS could potentially influence cell survival and death following the loss of afferent input. The primary rationale for pursuing the present study was that previous reports indicate a rapid increase in intracellular calcium (Zirpel et al., 1995) and a dramatic mitochondrial proliferation in NM following deafferentation (Hyde and Durham, 1994). It was then hypothesized that deafferentation will lead to a
Acknowledgments
This work was supported by NIH grant DC00858.
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