NeuroanatomyThe distribution of dopamine D1 receptor and μ-opioid receptor 1 receptor immunoreactivities in the amygdala and interstitial nucleus of the posterior limb of the anterior commissure: Relationships to tyrosine hydroxylase and opioid peptide terminal systems
Section snippets
Tissue preparation
All animal experiments were carried out in accordance with the requirements of the Canadian Council on Animal Care Guide (Policy 31, 1993), the Animals for Research Act (R.S.O., 1990, c.22) and the National Institutes of Health Guide for the Care and Use of Laboratory Animals (NIH Publication No. 80–23, revised 1996). All efforts were made to minimize the number of animals used and their suffering. Sprague–Dawley rats (n=6: Charles River, Wilmington, MA, USA) were anesthetized with sodium
Results
The architecture of the regions examined is schematically shown in Fig. 1, adapted from the atlas of Paxinos and Watson (1997). Note that rostro-caudally, these regions span up to 5 mm in the rat brain.
Mismatches in dopamine and opioid peptide systems
The present findings demonstrate transmitter–receptor mismatches in the MOR mediated ENK and β-endorphin transmission in distinct subregions of the amygdala and the extended amygdala. Furthermore, the present findings confirm and extend previous observations regarding such mismatches in dopaminergic neuronal systems in the amygdala (Fuxe et al., 2003).
The neurotransmitter–receptor mismatch phenomena have previously been extensively discussed and analyzed (Agnati et al 1986, Fuxe and Agnati 1991
Acknowledgments
Authors were supported by the Natural Sciences and Engineering Research Council of Canada and by a grant from the Swedish Research Council.
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