NeuroanatomyNeuronal localization of 5-HT type 2A receptor immunoreactivity in the rat basolateral amygdala
Section snippets
Tissue preparation
A total of 28 male Sprague–Dawley rats (250–350 g; Harlan, Indianapolis, IN, USA) were used in this study. All experiments were carried out in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals and were approved by the Institutional Animal Use and Care Committee (IACUC) of the University of South Carolina. All efforts were made to minimize animal suffering and to use the minimum number of animals necessary to produce reliable scientific data.
Rats
Results
The pattern of immunostaining obtained in the amygdala with the three 5-HT2AR antibodies was different (Fig. 1, Fig. 2, Fig. 3, Fig. 4, Fig. 5, Fig. 6, Fig. 7), and each will be described separately.
Discussion
Although there is evidence that the monoclonal and polyclonal antibodies used in this study are specific for 5-HT2AR (see above), their staining patterns in the BLC were different. The OC antibody mainly stained nonpyramidal interneurons that expressed PV or SOM, although numerous dendrite-like processes were stained in the Ldl. The density of the latter processes indicates that they probably arise from pyramidal cells, the principal neurons of the BLC. In contrast, the BD antibody appeared to
Acknowledgments
The authors would like to thank the following investigators for their donations of the antibodies used in this study: Dr. Kenneth Baimbridge (University of British Columbia) for the rabbit polyclonal antibody to parvalbumin, Dr. John H. Walsh (CURE/Digestive Diseases Research Center, Antibody/RIA Core, NIH grant #DK41301, Los Angeles, CA, USA) for the mouse anti-CCK antibody, Dr. Bryan Roth (University of North Carolina School of Medicine) for the Ab51 polyclonal 5-HT2AR antibody, and Dr.
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