Abstract
In heart tissue from mice lacking the intermediate filament (IF) desmin, mitochondria show an abnormal shape and distribution (Thornell et al., 1997). In the present study we have isolated heart mitochondria from desmin null (D−/−) and control (D+/+) mice, and analyzed their composition by SDS–PAGE, immunoblotting, and enzyme measurements. We found both in vitro and in situ that the conventional kinesin, the microtubule-associated plus-end directed motor, was frequently associated with D+/+ heart mitochondria, but not with D−/− heart mitochondria, suggesting that the positioning of mitochondria in heart is a dynamic event involving the IF desmin, the molecular motor kinesin, and, most likely, the microtubules (MT) network. Furthermore, an increased capacity in energy production was found, as indicated by a threefold higher creatine kinase activity in heart mitochondria from D−/− compared to D+/+ mice. We also observed a significantly lower amount of cytochrome c in heart mitochondria from D−/− mice, and a relocalization of Bcl-2, which may indicate an apoptotic condition in the cell leading to the earlier reported pathological events, such as cardiomyocytes degeneration and calcinosis of the heart (Thornell et al., 1997).
Similar content being viewed by others
REFERENCES
Abe-Dohmae, S., Harada, N., Yamada, K., and Tanaka, R. (1993). Biochem. Biophys. Res. Commun. 191, 915-921.
Ades, I. Z. and Cascarno, J. (1977). J. Bioenerg. Biomembr. 9, 237-253.
Andreyev, A. Y., Fahy, B., and Fiskum, G. (1998). FEBS Lett. 439, 373-376.
Bereiter-Hahn, J. and Vöth, M. (1994). Micros. Res. Technol. 27, 198-219.
Capetanaki, Y., Milner, D. J., and Weitzer, G. (1997). Cell. Struct. Function 22, 103-116.
Carlsson, L., Li, Z. L., Paulin, D., Price, M. G., Breckler, J., Robson, R. M., Wiche, G., and Thornell, L. E. (2000). Histochem. Cell. Biol. 114, 39-47.
Collier, N. C., Sheetz, M. P., and Schlesinger M. J. (1993). J. Cell. Biochem. 52, 297-397.
Cook, S. A., Sugden, P. H., and Clerk, A. (1999). Circ. Res. 85, 940-949.
Doran, E. and Halestrap, A. P. (2000). Biochem. J. 348, 343-350.
Elluru, R. G., Bloom, G. S., and Brady, S. T. (1995). Mol Biol Cell 6, 21-40.
Field, M. L., Clark, J. F., Henderson, C., Seymour, A. M., and Radda, G. K. (1994). Cardiovasc. Res. 28, 86-91.
Gomez-Cambronero, J., Huang, C. K., Gomez-Cambronero, T. M., Waterman, W. H., Becker, E. L., and Sha'afi, R. I. (1992). Proc. Natl. Acad. Sci. USA 89, 7551-7555.
Gotow, T., Sakata, M., Funakoshi, T., and Uchiyama, Y. (1996). Neuroscience 75, 507-521.
Gotow, T., Shibata, M., Kanamori, S., Tokuno, O., Ohsawa, Y., Sato, N., Isahara, K., Yayoi, Y., Watanabe, T., Leterrier, JF., Lindén, M., Kominami, E., and Uchiyama, Y. (2000). Cell Death Differ. 7, 666-674.
Gyoeva, F. K. and Gelfand, V. I. (1991). Nature (London) 353, 445-448.
Hughes, B. P. (1962). Clin. Chim. Acta 7, 579.
Ingold A. L., Cohn S. A., and Scholey J. M. (1988). J. Cell Biol. 107, 2657-2667.
Jellali, A., Metz-Boutigue, M. H., Surgucheva, I., Jancsik, V., Schwartz, C., Filliol, D., Gelfand, V. I., and Rendon, A. (1994). Cell. Motility Cytoskelton 28, 79-93.
Laemmli, U.K. (1970). Nature (London) 227, 680-685.
Lee, C. P., Martens, M. E., Jankulowska, L., and Neymark, M. A. (1979). Muscle Nerve 5, 340-348.
Leopold, P. L., McDowall, A. W., Pfister, K. K., Bloom, G. S., and Brady, S. T. (1992). Cell Motility Cytoskeleton 23, 19-33.
Leterrier, J. F., Rusakov, D. A., Nelson, B. D., and Lindén, M. (1994). J. Microsc. Res. Technol. 27, 233-261.
Li, Z., Colucci-Guyon E., Pinçon-Raymond, M., Mericskay, M., Pournin, S., Paulin, D., and Babinet, C. (1996). Develop. Biol. 175, 362-366.
Lindén, M., Andersson, G., Gellerfors, P., and Nelson, B. D. (1984). Biochim. Biophys. Acta 770, 93-96.
Lipskaya, T. Yu., Geiger, P. J., and Bessman, S. P. (1995). Biochem. Mol. Med. 55, 81-89.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951). J. Biol. Chem. 193, 265-275.
Milner, D. J., Mavroidis, M., Weisleder, N., and Capetanaki, Y. (2000). J. Cell. Biol. 150, 1283-1298.
Nangaku, M., Sato-Yoshitake, R., Okada, Y., Noda, Y., Takemura, R., Yamazaki, H., and Hirokawa, N. (1994). Cell 79, 1209-1220.
Ogata, T. and Yamasaki, Y. (1997). Anat. Rec. 248, 214-223.
Prahlad, V., Yoon, M., Moir, R. D., Vale, R. D., and Goldman, R. D. (1998). J. Cell. Biol. 143, 159-170.
Reipert, R., Steinbock, F., Fischer, I., Bittner, R. E., Zeold, A., and Wiche, G. (1999). Exp. Cell. Res. 252, 479-491.
Seccia, T. M., Atlante, A., Vulpis, V., Marra, E., Passarella, S., and Pirelli, A. (1998). Clin. Exp. Hypertension 20, 345-358.
Shimizu, S., Narita, M., and Tsujimoto, Y. (1999). Nature (London) 399, 483-487.
Soltys B. J. and Gupta, R. S. (1992). Biochem. Cell. Biol. 70, 1174-1186.
Stromer, M. H. and Bendayan, M. (1988). Cell Motility Cytoskeleton 11, 117-125.
Stromer, M. H. and Bendayan, M. (1990). Cell Motility Cytoskeleton 17, 11-18.
Thornell, L. E., Carlsson, L., Li, Z., Mericskay, M., and Paulin D. (1997). J. Mol. Cell Cardiol. 29, 2107-2124.
Tokuyasu K. T. (1980). Histochem. J. 12, 381-403.
Tokuyasu, K. T., Dutton, A. H., and Singer, S. J. (1983a). J. Cell Biol. 96, 1727-1735.
Tokuyasu, K. T., Dutton, A. H., and Singer, S. J. (1983b). J. Cell Biol. 96, 1736-1742.
Towbin, H., Staehlin, T., and Gordon, J. (1979). Proc. Natl. Acad. Sci. USA 76, 4350-4354.
von Ahsen, O., Renken, C., Perkins, G. Kluck, R. M., Bossy-Wetzel, E., and Newmeyer, D. D. (2000). J. Cell. Biol. 150, 1027-1036.
Watkins, S. C., Samuel, J. L., Marotte, F., Bertier-Savalle, B., and Rappaport, L. (1987). Circ. Res. 60, 327-336.
Yabe, J. T., Pimenta, A., and Shea, T. B. (1999). J. Cell. Sci. 112, 3799-3814.
Rights and permissions
About this article
Cite this article
Lindén, M., Li, Z., Paulin, D. et al. Effects of Desmin Gene Knockout on Mice Heart Mitochondria. J Bioenerg Biomembr 33, 333–341 (2001). https://doi.org/10.1023/A:1010611408007
Issue Date:
DOI: https://doi.org/10.1023/A:1010611408007