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Neural cell fate analysis in zebrafish using olig2 BAC transgenics

  • Published:
Methods in Cell Science

Abstract

We describe here simple methods for producing transgenic zebrafish reporter lines using BAC clones. The use of BAC clones facilitates creation of useful transgenics as the large amounts of genomic DNA they contain increase the likelihood that reporter gene expression will be properly regulated. Combined with recent advances in live embryo image analysis, this strategy has the potential to greatly advance the investigation of neural cell behavior during development.

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Authors and Affiliations

  1. Department of Biological Sciences, Vanderbilt University, Nashville, TN, 37232, USA

    Jimann Shin, Hae-Chul Park, Jolanta M. Topczewska & David J. Mawdsley

  2. Department of Biological Sciences, Vanderbilt University, 2401 Stevenson Center, 1161 21st Avenue South, Nashville, TN, 37232, USA

    Bruce Appel

Authors
  1. Jimann Shin
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  2. Hae-Chul Park
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  3. Jolanta M. Topczewska
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  4. David J. Mawdsley
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  5. Bruce Appel
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Shin, J., Park, HC., Topczewska, J.M. et al. Neural cell fate analysis in zebrafish using olig2 BAC transgenics. Methods Cell Sci 25, 7–14 (2003). https://doi.org/10.1023/B:MICS.0000006847.09037.3a

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  • DOI: https://doi.org/10.1023/B:MICS.0000006847.09037.3a

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