Gating of a muscle K⁺ channel and its dependence on the permeating ion species

Abstract

In excitable cells, ions permeate the cell membrane through ionic channels, some of which open and close in response to changes in the potential difference across the membrane. It has been supposed that this opening and closing (or gating) process is largely independent of the permeating ion. However, we show here that the gating of the resting potassium permeability of frog skeletal muscle depends on the species of ion which carries current across the membrane. The potassium permeability investigated allows K⁺ to move in across the membrane more easily than out^1,2. This property is known as inward or anomalous rectification and is shared by cell membranes of skeletal muscle^1–3, egg⁴ and certain other cells^5–7. In both egg cells⁸ and skeletal muscle fibres⁹, the group IIIB metal ion T1⁺, which can replace K⁺ in several other systems in experimental conditions^10–12, also permeates the inward rectifier. Indeed, Tl⁺ is more permeant than K⁺ (refs 8, 9). However, when Tl⁺ carries current inwards across the membrane, the inward rectifier inactivates over a brief period when the membrane is hyper-polarized, whereas when K⁺ carries current, the permeability increases with time under hyperpolarization.