Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Brain spectrin, a membrane-associated protein related in structure and function to erythrocyte spectrin

Nature volume 299, pages 126–131 (1982)Cite this article

Abstract

An immunoreactive analogue of erythrocyte spectrin has been purified from brain membranes. This protein co-sediments with and cross-links actin filaments, associates with spectrin-binding sites on erythrocyte membranes, and has been visualized by rotary shadowing as an extended, flexible rod. The brain spectrin comprises 3% of the total membrane protein, and may have a major role in mediating linkage of actin to membranes.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Weihing, R. B. Meth. Achieve. exp. Path. 8, 42–109 (1979).

    CAS  Google Scholar 

  2. Koch, G. & Smith, M. Nature 273, 274–278 (1978).

    Article  ADS  CAS  PubMed  Google Scholar 

  3. Flanagan, J. & Koch, G. Nature 273, 278–281 (1978).

    Article  ADS  CAS  PubMed  Google Scholar 

  4. Ohnishi, T. J. Biochem. 52, 307–308 (1963).

    Article  Google Scholar 

  5. Tilney, L. G. & Detmers, P. J. Cell Biol. 66, 508–520 (1975).

    Article  CAS  PubMed  Google Scholar 

  6. Branton, D., Cohen, C. M. & Tyler, J. Cell 24, 24–32 (1981).

    Article  CAS  PubMed  Google Scholar 

  7. Bennett, V. J. cell. Biochem. 18, 49–66 (1982).

    Article  CAS  PubMed  Google Scholar 

  8. Brenner, S. L. & Korn, E. D. J. biol. Chem. 254, 8620–8627 (1979).

    CAS  PubMed  Google Scholar 

  9. Ungewickell, E., Bennett, P. M., Calvert, R., Ohanian, V. & Gratzer, W. Nature 280, 811–816 (1979).

    Article  ADS  CAS  PubMed  Google Scholar 

  10. Fowler, V. & Taylor, D. L. J. Cell Biol. 85, 361–376 (1980).

    Article  CAS  PubMed  Google Scholar 

  11. Shotton, D. M., Burke, B. E. & Branton, D. J. molec. Biol. 131, 303–329 (1979).

    Article  CAS  PubMed  Google Scholar 

  12. Bennett, V. & Stenbuck, P. J. biol. Chem. 254, 2533–2541 (1979).

    CAS  PubMed  Google Scholar 

  13. Luna, E., Kidd, G. & Branton, D. J. biol. Chem. 254, 2526–2532 (1979).

    CAS  PubMed  Google Scholar 

  14. Yu, J. & Goodman, S. Proc. natn. Acad. Sci. U.S.A. 76, 2340–2344 (1979).

    Article  ADS  CAS  Google Scholar 

  15. Calvert, R., Bennett, P. & Gratzer, W. Eur. J. Biochem. 107, 355–361 (1980).

    Article  CAS  PubMed  Google Scholar 

  16. Litman, D., Hsu, C. J. & Marchesi, V. T. J. Cell Sci. 42, 1–22 (1980).

    CAS  PubMed  Google Scholar 

  17. Bennett, V. & Stenbuck, P. Nature 280, 468–473 (1979).

    Article  ADS  CAS  PubMed  Google Scholar 

  18. Bennett, V. & Stenbuck, P. J. biol. Chem. 255, 2540–2548 (1980).

    CAS  PubMed  Google Scholar 

  19. Hargreaves, W. R., Giedd, K. M., Verkleij, A. & Branton, D. J. biol. Chem. 255, 11965–11972 (1980).

    CAS  PubMed  Google Scholar 

  20. Hiller, G. & Weber, K. Nature 266, 181–183 (1977).

    Article  ADS  CAS  PubMed  Google Scholar 

  21. Goodman, S., Zagon, I. S. & Kulikowski, R. Proc. natn. Acad. Sci. U.S.A. 78, 7570–7574 (1981).

    Article  ADS  CAS  Google Scholar 

  22. Davis, J. & Bennett, V. J. biol. Chem. 257, 5816–5820 (1982).

    CAS  PubMed  Google Scholar 

  23. Bennett, V. Nature 281, 597–599 (1979).

    Article  ADS  CAS  PubMed  Google Scholar 

  24. Bennett, V. & Davis, J. Proc. natn. Acad. Sci. U.S.A. 78, 7550–7554 (1981).

    Article  ADS  CAS  Google Scholar 

  25. Sattilaro, R. F., Dentler, W. L. & LeCluyse, L. J. Cell Biol. 90, 467–473 (1981).

    Article  CAS  PubMed  Google Scholar 

  26. Voter, W. A. & Erickson, H. P. J. Cell Biol. 91, 331a (1981).

    Google Scholar 

  27. Tyler, J. M., Reinhardt, B. & Branton, D. J. biol. Chem. 255, 7034 (1980).

    CAS  PubMed  Google Scholar 

  28. Morrow, J., Speicher, D., Knowles, W., Hsu, C. & Marchesi, V. T. Proc. natn. Acad. Sci. U.S.A. 77, 6592–6597 (1981).

    Article  ADS  Google Scholar 

  29. Bennett, V. J. biol. Chem. 253, 2292–2299 (1978).

    CAS  PubMed  Google Scholar 

  30. Shimo-Oka, T. & Watanabe, Y. J. Biochem. 90, 1297–1307 (1981).

    Article  CAS  PubMed  Google Scholar 

  31. Levine, J. & Willard, M. J. Cell Biol. 90, 631–643 (1981).

    Article  CAS  PubMed  Google Scholar 

  32. Bennett, V., Davis, J. & Fowler, W. E. Phil. Trans. R. Soc. B (in the press)

  33. Luna, E., Fowler, V., Swanson, J., Branton, D. & Taylor, D. L. J. Cell Biol. 88, 396–409 (1981).

    Article  CAS  PubMed  Google Scholar 

  34. Mescher, M., Jose, M. & Balk, S. Nature 289, 139–144 (1981).

    Article  ADS  CAS  PubMed  Google Scholar 

  35. Schliwa, M. & van Blerkom, J. J. Cell Biol. 90, 222–235 (1981).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  36. Gulley, R. L. & Reese, T. S. J. Cell Biol. 91, 298–302 (1981).

    Article  CAS  PubMed  Google Scholar 

  37. Glenney, J., Glenney, P. & Weber, K. Cell 28, 843–854 (1982).

    Article  CAS  PubMed  Google Scholar 

  38. Repasky, E., Granger, B. & Lazarides, E. Cell 29, 821–833 (1982).

    Article  CAS  PubMed  Google Scholar 

  39. Burridge, K., Kelly, T. & Connel, L. Phil. Trans. R. Soc. B (in the press).

  40. Toda, G., Oka, H., Oda, T. & Ikeda, Y. Biochim. biophys. Acta 413, 52–64 (1975).

    Article  CAS  PubMed  Google Scholar 

  41. Fleischer, S. & Kervina, M. Meth. Enzym. 31A, 15 (1974).

    Google Scholar 

  42. Dwyer, N. & Blobel, G. J. Cell Biol. 70, 581–591 (1976).

    Article  CAS  PubMed  Google Scholar 

  43. Elder, J. H., Pickett, R. A., Hampton, J. & Lerner, R. A. J. biol. Chem. 252, 6510–6516 (1980).

    Google Scholar 

  44. Fowler, W. E. & Erickson, H. P. J. molec. Biol. 134, 241–249 (1979).

    Article  CAS  PubMed  Google Scholar 

  45. Bennett, V. & Branton, D. J. biol. Chem. 252, 2753–2763 (1977).

    CAS  PubMed  Google Scholar 

  46. Spudich, J. A. & Watt, S. J. J. biol. Chem. 246, 4866–4871 (1971).

    CAS  PubMed  Google Scholar 

  47. MacLean-Fletcher, S. & Pollard, T. D. Biochem. biophys. Res. Commun. 96, 18–27 (1980).

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Cell Biology and Anatomy, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, Maryland, 21205, USA

    Vann Bennett, Jonathan Davis & Walter E. Fowler

Authors
  1. Vann Bennett
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jonathan Davis
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Walter E. Fowler
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bennett, V., Davis, J. & Fowler, W. Brain spectrin, a membrane-associated protein related in structure and function to erythrocyte spectrin. Nature 299, 126–131 (1982). https://doi.org/10.1038/299126a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/299126a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing