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Phosphorylation of elongation factor 2 by EF-2 kinase affects rate of translation

Abstract

A new Ca2+/calmodulin-dependent protein kinase has been recently discovered in mammalian cells1,2. The major substrate of this kinase, a protein of relative molecular mass (Mr) ≈100,000 (100K), has been identified as elongation factor 2 (EF-2)3,4, which participates in protein synthesis. The in vivo activity of the EF-2 kinase depends upon growth factors and other agents affecting the level of Ca2+ and cAMP5,6. Its effect on EF-2 activity, however, remained obscure. This work shows that the phosphorylation of EF-2 by the EF-2 kinase results in a drastic inhibition of polyphenylalanine synthesis in poly(U)-directed translation. Phosphorylated EF-2 is completely inactive in translation and, moreover, inhibits the activity of non-phosphorylated EF-2. Dephosphorylation of EF-2 by phosphatase restores its activity. Hence, the phosphorylation of EF-2 directly affects the elongation stage of translation and thus represents a novel mechanism of translational control.

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References

  1. Palfrey, H. C. FEBS Lett. 157, 183–190 (1983).

    Article  CAS  Google Scholar 

  2. Nairn, A. C., Bhagat, B. & Palfrey, H. C. Proc. natn. Acad. Sci. U.S.A. 82, 7939–7943 (1985).

    Article  ADS  CAS  Google Scholar 

  3. Ryazanov, A. G. FEBS Lett. 214, 331–334 (1987).

    Article  CAS  Google Scholar 

  4. Ryazanov, A. G., Natapov, P. G., Shestakova, E. A., Severin, F. F. & Spirin, A. S. Biochimie (in the press).

  5. Palfrey, H. C., Nairn, A. C., Muldoon, L. L. & Villereal, M. L. J. biol. Chem. 262, 9785–9792 (1987).

    CAS  PubMed  Google Scholar 

  6. Nairn, A. C., Nichols, R. A., Brady, M. J. & Palfrey, H. C. J. biol. Chem. 262, 14265–14272 (1987).

    CAS  PubMed  Google Scholar 

  7. Pain, V. M. Biochem. J. 235, 625–637 (1986).

    Article  CAS  Google Scholar 

  8. Sitikov, A. S., Simonenko, P. N., Shestakova, E. A., Ryazanov, A. G. & Ovchinnikov, L. P. FEBS Lett. 228, 327–331 (1988).

    Article  CAS  Google Scholar 

  9. Shestakova, E. A. & Ryazanov, A. G. Dokl. Akad. Nauk SSSR 297, 1495–1498 (1987).

    CAS  PubMed  Google Scholar 

  10. Hashimoto, S., Iwasaki, C., Kuzuya, H. & Guroff, G. J. Neurochem. 46, 1599–1604 (1986).

    Article  CAS  Google Scholar 

  11. Nairn, A. C. & Palfrey, H. C. J. biol. Chem. 262, 17299–17303 (1987).

    CAS  PubMed  Google Scholar 

  12. O'Farrell, P. H. J. biol. Chem. 250, 4007–4021 (1975).

    CAS  PubMed  PubMed Central  Google Scholar 

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Ryazanov, A., Shestakova, E. & Natapov, P. Phosphorylation of elongation factor 2 by EF-2 kinase affects rate of translation. Nature 334, 170–173 (1988). https://doi.org/10.1038/334170a0

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