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Assignment of G-protein subtypes to specific receptors inducing inhibition of calcium currents

Abstract

The inhibition of voltage-dependent Ca2+ channels in secretory cells by plasma membrane receptors is mediated by pertussis toxin-sensitive G proteins. Multiple forms of G proteins have been described, differing principally in their α subunits, but it has not been possible to establish which G-protein subtype mediates inhibition by a specific receptor. By intranuclear injection of antisense oligonucleotides into rat pituitary GH3 cells, the essential role of the Go-type G proteins in Ca2+-channel inhibition is established: the subtypes Go1 and Go2 mediate inhibition through the muscarinic and somatostatin receptors, respectively.

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Kleuss, C., Hescheler, J., Ewel, C. et al. Assignment of G-protein subtypes to specific receptors inducing inhibition of calcium currents. Nature 353, 43–48 (1991). https://doi.org/10.1038/353043a0

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