Abstract
A KEY event in the response of cells to proliferative signals is the rapid, transient induction of the c-fos proto-oncogene, which is mediated through the serum response element (SRE) in the fos promoter1–4. Genomic footprinting5,6 and transfection experi-ments7–9 suggest that this activation occurs through a ternary complex that includes the serum response factor (SRF)10 and the ternary complex factor p62 (ref. 7). Interaction of p62TCF with the SRF-SRE binary complex requires a CAGGA tract immediately upstream of the SRE (ref. 7). Proteins of the ets proto-oncogene family bind to similar sequences11–13and we have found that a member of this family, Elk-1 (ref. 14), forms SRF-dependent ternary complexes with the SRE. Elk-1 and p62TCF have the same DNA sequence requirements and antibodies against Elk-1 block the binding of both proteins. Furthermore, we show that like p62TCF, Elk-1 forms complexes with the yeast SRF-homologue MCM1 but not with yeast ARG80 (ref. 15). But ARG80 mutants that convey interaction with p62TCF can also form complexes with Elk-1. The similarity, or even identity, between Elk-1 and p62TCF suggests a novel regulatory role for Ets proteins that is effected through interaction with other proteins, such as SRF. Furthermore, the possible involvement of an Ets protein in the control of c-fos has interesting implications for proto-oncogene cooperation in cellular growth control16.
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References
Greenberg, M. E. & Ziff, E. B. Nature 311, 433–438 (1984).
Treisman, R. Cell 42, 889–902 (1985).
Gilman, M. Z., Wilson, R. N. & Weinberg, R. A. Molec. cell. Biol. 6, 4305–4314 (1986).
Treisman, R. Cancer Biol. 1, 47–58 (1990).
Herrera, R. E., Shaw, P. E. & Nordheim, A. Nature 340, 68–70 (1989).
König, H. Nucleic Acids Res. 19, 3607–3611 (1991).
Shaw, P. E., Schröter, H. & Nordheim, A. Cell 56, 563–572 (1989).
Graham, R. & Gilman, M. Science 251, 189–192 (1991).
Gutman, A., Wasylyk, C. & Wasylyk, B. Molec. cell. Biol. 11, 5381–5387 (1991).
Treisman, R. Cell 46, 567–574 (1986).
Karim, F. D. et al. Genes Dev. 4, 1451–1453 (1990).
Rao, V. N. & Reddy, E. S. P. Oncogene (in the press).
Reddy, E. S. P. & Rao, V. N. Oncogene (in the press).
Rao, V. N. et al. Science 244, 66–70 (1989).
Mueller, C. G. F. & Nordheim, A. EMBO J. (in the press).
Gutman, A. & Wasylyk, B. Trends Genet. 7, 49–54 (1991).
Norman, C., Runswick, M., Pollock, R. M. & Treisman, R. Cell 55, 989–1003 (1988).
Schröter, H., Mueller, C. G. F., Meese, K. & Nordheim, A. EMBO J. 9, 1123–1130 (1990).
Watson, D. K. et al. Proc. natn. Acad. Sci. U.S.A. 85, 7862–7866 (1988).
Reddy, E. S. P. & Rao, V. N. Cancer Res. 50, 5013–5016 (1990).
Dolan, J. W. & Fields, S., Biochim. biophys. Acta. 1088, 155–169 (1991).
Wasylyk, B. et al. Nature 346, 191–193 (1990).
Bosselut, R. et al. EMBO J. 9, 3137–3144 (1990).
Gunther, C. V., Nye, J. A., Bryer, R. S. & Graves, B. J. Genes Dev. 4, 667–679 (1990).
Thompson, C. C., Brown, T. A. & McKnight, S. L. Science 253, 762–768 (1991).
Turc-Carel, C. et al. Proc. natn. Acad. Sci. U.S.A. 84, 1981–1985 (1987).
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Hipskind, R., Roa, V., Muller, C. et al. Ets-related protein Elk-1 is homologous to the c-fos regulatory factor p62TCF. Nature 354, 531–534 (1991). https://doi.org/10.1038/354531a0
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DOI: https://doi.org/10.1038/354531a0
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