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Two sodium channels contribute to the TTX-R sodium current in primary sensory neurons

Abstract

We have cloned a second tetrodotoxin-resistant (TTX-R) sodium channel α subunit, SNS2, with the same amino-acid sequence as a putative sodium channel α subunit NaN (ref. 1). SNS2 expression in HEK293T cells produced a TTX-R voltage-gated sodium current with faster kinetics and a lower TTX IC50 than the previously cloned sensory-neuron-specific sodium channel, SNS/PN3 (Refs 2, 3). SNS2 was co-expressed with SNS/PN3 in small DRG neurons.

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Figure 1: Localization of SNS2 and SNS/PN3 in subsets of DRG neurons.
Figure 2: Distribution of SNS2 and SNS/PN3 in development and following chronic inflammation or nerve injury.
Figure 3: Biophysical and pharmacological properties of recombinant rat SNS/PN3 (left panels) and SNS2 (right panels) sodium channels expressed in HEK293T cells.

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Acknowledgements

C.J.W. was supported by MRC G9431792, HFSP RG73/96 and Wellcome Trust 039614. We thank Inger Selmer for her initial involvement in this project.

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Correspondence to Simon Tate.

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Tate, S., Benn, S., Hick, C. et al. Two sodium channels contribute to the TTX-R sodium current in primary sensory neurons. Nat Neurosci 1, 653–655 (1998). https://doi.org/10.1038/3652

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