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Ether-à-go-go encodes a voltage-gated channel permeable to K+ and Ca2+ and modulated by cAMP

Nature volume 365, pages 445–448 (1993)Cite this article

Abstract

THE Drosophila ether-à-go-go (eag) mutant is responsible for altered potassium currents in excitable tissue1. These mutants exhibit spontaneous, repetitive firing of action potentials in the motor axons of larval neuromuscular junctions2. The eag gene encodes a polypeptide3 that shares sequence similarities with several different ionic channel proteins, including voltage-gated potassium channels3, an inward rectifier4,5 as well as cyclic-nucleotide-gated channels6. These formal similarities in the derived primary sequences indicate that eag polypeptides might express a new type of ion channel. Here we report the expression by eag RNA in Xenopus oocytes of such a channel which incorporates properties of both voltage- and ligand-gated channels. The perme-ability of these eag channels to potassium and calcium is dependent on voltage and cyclic AMP. The ability to mediate potassium-outward and calcium-inward currents endows this channel with properties likely to be important in the modulation of synaptic efficiency in both central and peripheral nervous systems.

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References

  1. Zhong, Y. & Wu, C.-F. Science 252, 1562–1564 (1991).

    Article  ADS  CAS  Google Scholar 

  2. Ganetzky, B. & Wu, C.-F. A. Rev. Genet. 20, 13–44 (1986).

    Article  CAS  Google Scholar 

  3. Warmke, J., Drysdale, R. & Ganetzky, B. Science 252, 1560–1562 (1991).

    Article  ADS  CAS  Google Scholar 

  4. Sentnac, H. et al. Science 256, 663–665 (1992).

    Article  ADS  Google Scholar 

  5. Anderson, J. A., Huprikar, S. S., Kochian, L. V., Lucas, W. J. & Gaber, R. F. Proc. natn. Acad. Sci. U.S.A. 89, 3736–3740 (1992).

    Article  ADS  CAS  Google Scholar 

  6. Guy, H. R., Durrell, S. R., Warmke, J., Drysdale, R. & Ganetzky, B. Science 254, 730–730 (1991).

    Article  ADS  CAS  Google Scholar 

  7. Heinemann, S. H. & Conti, F. Meth. Enzym. 207, 131–148 (1992).

    Article  CAS  Google Scholar 

  8. Pongs, O. Trends pharmac. Sci. 13, 359–365 (1992).

    Article  CAS  Google Scholar 

  9. Pardo, L. A. et al. Proc. natn. Acad. Sci. U.S.A. 89, 2466–2470 (1992).

    Article  ADS  CAS  Google Scholar 

  10. López-Barneo, J., Hoshi, T., Heinemann, S. H. & Aldrich, R. Receptors and Channels 1, 61–71 (1993).

    PubMed  Google Scholar 

  11. Eisenman, G. Biophys. J. 2, 259–323 (1962).

    Article  ADS  CAS  Google Scholar 

  12. White, M. M. & Aylwin, M. Molec. Pharmac. 37, 720–724 (1990).

    CAS  Google Scholar 

  13. Lory, P., Rassendren, F. A., Richard, S., Tiaho, F. & Nargeot, J. J. Physiol., Lond. 429, 95–112 (1990).

    Article  CAS  Google Scholar 

  14. Wu, C.-F., Ganetzky, B., Haugland, F. N. & Liu, A. X. Science 220, 1076–1078 (1983).

    Article  ADS  CAS  Google Scholar 

  15. Zagotta, W. N., Gemeraad, S., Garber, S., Hoshi, T. & Aldrich, R. W. Neuron 3, 773–782 (1989).

    Article  CAS  Google Scholar 

  16. Jan, L. Y. & Jan, Y. N. Nature 345, 672 (1990).

    Article  ADS  CAS  Google Scholar 

  17. Heginbotham, L., Abramson, T. & MacKinnon, R. Science 258, 1152–1155 (1992).

    Article  ADS  CAS  Google Scholar 

  18. Pongs, O. et al. EMBO J. 7, 1087–1096 (1988).

    Article  CAS  Google Scholar 

  19. Sambrook, J., Fritsch, E. F. & Maniatis, T. in Molecular Cloning: A Laboratory Manual (2nd edn) (Cold Spring Harbor Laboratory Press, New York, 1989).

    Google Scholar 

  20. Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).

    Article  ADS  CAS  Google Scholar 

  21. Stacker, M. et al. Proc. R. Soc. B 245, 101–107 (1991).

    Article  ADS  Google Scholar 

  22. Toro, L., Ottolia, M., Olcese, R. & Stefani, E. Biophys. J. 64, A2 (1993).

    Article  Google Scholar 

  23. Stühmer, W. Meth. Enzym. 207, 319–339 (1992).

    Article  Google Scholar 

Download references

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Author notes

  1. Walter Stühmer: To whom correspondence should be addressed.

Authors and Affiliations

  1. Max-Planck-lnstitut fur experimentelle Medizin, Hermann-Rein-Strasse 3, D-37075, Gottingen, Germany

    Andrea Brüggemann, Luis A. Pardo & Walter Stühmer

  2. ZMNH, Institut fur Neuronale Signalverarbeitung, Martinistrasse 52, D-20246, Hamburg, Germany

    Andrea Brüggemann & Olaf Pongs

  3. Departamento de Biologia Funcional, Universidad de Oviedo, E-33006, Oviedo, Spain

    Luis A. Pardo

Authors
  1. Andrea Brüggemann
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  2. Luis A. Pardo
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  3. Walter Stühmer
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  4. Olaf Pongs
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Brüggemann, A., Pardo, L., Stühmer, W. et al. Ether-à-go-go encodes a voltage-gated channel permeable to K+ and Ca2+ and modulated by cAMP. Nature 365, 445–448 (1993). https://doi.org/10.1038/365445a0

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