Abstract
NEUROTRANSMITTER release is frequently regulated by peptides that modulate neuronal calcium channels. Whole-cell recordings show that the ion permeability1 and voltage dependence2 of these channels are controlled by a membrane-associated pathway involv-ing GTP-binding proteins. Here we use perforated-patch recordings to show that, in addition to this pathway, the peptide somatostatin inhibits the calcium current in chick ciliary ganglion neurons by a second soluble pathway involving a cyclic GMP-dependent protein kinase (cGMP-PK). This somatostatin inhibi-tion of Ca2+ current did not desensitize and was not characterized by the slowing of Ca2+-current activation (kinetic slowing) observed in whole-cell recordings. When cGMP-PK was inhibited, somatostatin inhibition of Ca2+ current resembled that observed with whole-cell recordings. cGMP agonists mimic the effect of somatostatin only in perforated patch recordings. An endogenous cGMP-PK therefore forms part of the mechanism by which soma-tostatin induces a sustained inhibition of neuronal calcium channels.
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References
Kuo, C.-C. & Bean, B. P. Nature 365, 258–262 (1993).
Bean, B. P. Nature 340, 153–155 (1989).
Lewis, D. L., Weight, F. F. & Luini, A. Proc. natn. Acad. Sci. U.S.A. 83, 9035–9039 (1986).
Ikeda, S. R. & Schofield, G. G. J. Physiol., Lond. 409, 221–240 (1989).
Sah, D. W. Y. J. Neurosci. 10, 136–141 (1990).
Shapiro, M. S. & Hille, B. Neuron 10, 11–20 (1993).
Golard, A. & Siegelbaum, S. A. J. Neurosci. 13, 3884–3894 (1993).
Wang, H.-L., Reisine, T. & Dichter, M. Neuroscience 38, 335–342 (1990).
Dryer, S. E., Dourado, M. M. & Wisgirda, M. E. Neuroscience 44, 663–672 (1991).
Butt, E., Van Bemmelen, M., Fischer, L., Walter, U. & Jastorff, B. FEBS Lett. 263, 47–50 (1990).
Ashkenazi, A., Winslow, J. W., Peralta, E. G. & Peterson, G. L. Science 238, 272–275 (1987).
Mattera, R. et al. Science 243, 804–807 (1989).
Marchetti, C., Carbone, E. & Lux, H. D. Pflugers Arch. 406, 104–111 (1986).
Wanke, E., Ferroni, A., Ambrosini, A., Pozzan, T. & Meldolesi, J. Proc. natn. Acad. Sci. U.S.A. 84, 4313–4321 (1987).
Grassi, F. & Lux, H. D. Neurosci. Lett. 105, 113–119 (1989).
Kasai, H. & Aosaki, T. Pflugers Arch. 414, 145–149 (1989).
Elmsie, K. S., Zhou, W. & Jones, S. W. Neuron 5, 75–80 (1990).
Bley, K. R. & Tsien, R. W. Neuron 2, 379–391 (1990).
Diverse-Pierluissi, M. & Dunlap, K. Neuron 10, 753–760 (1993).
Hille, B. Neuron 9, 187–195 (1992).
Penington, N. J., Kelly, J. S. & Fox, A. P. J. Neurosci. 11, 3594–3609 (1991).
Yatani, A. et al. Science 238, 1288–1292 (1987).
Gray, D. B., Zelazny, D., Manthay, N. & Pilar, G. J. Neurosci. 10, 2687–2698 (1990).
Wentzek, L. A. F., Bowers, C. W., Khairallah, L. & Pilar, G. J. Neurosci. 13, 3143–3154 (1993).
Rae, J., Cooper, K., Gates, P. & Watsky, M. J. Neurosci. Meth. 37, 15–26 (1991).
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Meriney, S., Gray, D. & Pilar, G. Somatostatin-induced inhibition of neuronal Ca2+ current modulated by cGMP-dependent protein kinase. Nature 369, 336–339 (1994). https://doi.org/10.1038/369336a0
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DOI: https://doi.org/10.1038/369336a0
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