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Adenoviral vector-mediated rescue of the OMP-null phenotype in vivo

Nature Neuroscience volume 3, pages 1113–1120 (2000)Cite this article

Abstract

The use of gene deletion by homologous recombination to determine gene or protein function has wide application in vertebrate neurobiology. An ideal complement to gene deletion would be subsequent gene replacement to demonstrate re-acquisition of function. Here we used an adenoviral vector to replace the olfactory marker protein (OMP) gene in olfactory receptor neurons of adult OMP-null mice and demonstrated the subsequent re-acquisition of function. Our results show that short-term expression of OMP restores the kinetics of electrophysiological responses of OMP-null mice to those of the control phenotype. This adenoviral-mediated rescue of the OMP-null phenotype is consistent with involvement of OMP in olfactory transduction.

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Figure 1: Cells infected with the recombinant CMV-OMP-IRES-EGFP-AdV expressed both OMP and EGFP.
Figure 2: Representative EOG traces from control, OMP-null and rescued OMP-null mice.
Figure 3: Summary of EOG data showing onset and recovery kinetics of odor responses and the recovery from adaptation in control, OMP-null and adenovirus-infected mice.
Figure 4: The effect of infection rate and OMP levels on EOG responses.

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Acknowledgements

We thank P. Mombaerts for providing the pBSK-IRES-EGFP clone, S. Hardy for providing shuttle vector pAdlox and CRE8 cell line and K. Moriyoshi for providing CMV-EGFP-AdV. We thank Matthew Rogers and Dong-Jing Zou for suggestions on the manuscript. This research was supported by NIH grant DC R01-03112 (F.L.M.).

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Authors and Affiliations

  1. Department of Biological Sciences, 923 Fairchild, MC 2438

    Lidija Ivic & Stuart Firestein

  2. Columbia University, New York, 10027, New York, USA

    Lidija Ivic & Stuart Firestein

  3. Department of Anatomy and Neurobiology, University of Maryland, School of Medicine, HSF 280, 685 West Baltimore Street, Baltimore, 21201, Maryland, USA

    Martina M. Pyrski, Joyce W. Margolis, Linda J. Richards & Frank L. Margolis

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  1. Lidija Ivic
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Correspondence to Stuart Firestein.

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Ivic, L., Pyrski, M., Margolis, J. et al. Adenoviral vector-mediated rescue of the OMP-null phenotype in vivo. Nat Neurosci 3, 1113–1120 (2000). https://doi.org/10.1038/80632

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