Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

The rise of the 'projectome'

A Corrigendum to this article was published on 01 July 2007

Although new super-resolution imaging techniques provide valuable biological insights, some applications, such as determining the organization of neural projections in the brain, are better served by comprehensive imaging of very large samples at lower resolution.

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Figure 1: A comparison of imaging methods based on their resolving ability and the magnitude of the resulting data set created from imaging a cubic millimeter sample of mouse brain, indicated by the box in the image.

References

  1. Donnert, G. et al. Biophys J.; published online 16 February 2007.

  2. Gustafsson, M.G. Proc. Natl. Acad. Sci. USA 102, 13081–13086 (2005).

    Article  CAS  Google Scholar 

  3. Betzig, E. et al. Science 313, 1642–1645 (2006).

    Article  CAS  Google Scholar 

  4. Rust, M.J., Bates, M. & Zhuang, X. Nat. Methods 3, 793–795 (2006).

    Article  CAS  Google Scholar 

  5. Denk, W. & Horstmann, H. PLoS Biol. 2, e329 (2004).

    Article  Google Scholar 

  6. Verveer, P.J. et al. Nat. Methods 4, 311–313 (2007).

    Article  CAS  Google Scholar 

  7. Sharpe, J. et al. Science 296, 541–545 (2002).

    Article  CAS  Google Scholar 

  8. Huisken, J., Swoger, J., Del Bene, F., Wittbrodt, J. & Stelzer, E.H. Science 305, 1007–1009 (2004).

    Article  CAS  Google Scholar 

  9. Dodt, H.-U. et al. Nat. Methods 4, 331–336 (2007).

    Article  CAS  Google Scholar 

  10. Spalteholz, W. Über das Durchsichtigmachen von menschlichen und tierischen Präparaten. (S. Hierzel, Leipzig, 1914).

  11. Siedentopf, H. & Zsigmondy, R. Annalen der Physik 10, 1–39 (1903).

    CAS  Google Scholar 

  12. Feng, G. et al. Neuron 28, 41–51 (2000).

    Article  CAS  Google Scholar 

  13. Scott, E.K. et al. Nat. Methods 4, 323–326 (2007).

    Article  CAS  Google Scholar 

  14. Lein, E.S. et al. Nature 445, 168–176 (2007).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kasthuri, N., Lichtman, J. The rise of the 'projectome'. Nat Methods 4, 307–308 (2007). https://doi.org/10.1038/nmeth0407-307

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/nmeth0407-307

This article is cited by

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing