Noninvasive diode laser activation of transient receptor potential proteins and nociceptors

Nan Jiang; Brian Y. Cooper; Michael I. Nemenov

doi:10.1117/12.699204

21 February 2007 Noninvasive diode laser activation of transient receptor potential proteins and nociceptors

Nan Jiang, Brian Y. Cooper, Michael I. Nemenov

Proceedings Volume 6428, Mechanisms for Low-Light Therapy II; 642809 (2007) https://doi.org/10.1117/12.699204
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

We investigated diode laser (980 nm) evoked activation of transient receptor potential proteins (TRPV1 and TRPV2). C and A-delta (A&dgr;) nociceptor families are primarily responsible for pain mediation in the peripheral nervous system. TRPV1 proteins have been associated with heat evoked pain in C fibers while A&dgr; fibers have been associated with TRPV2. Diode laser stimulation allows a margin of safety between non-invasive activation and damage ^{19, 22, 34}. Laser pulses (20-50 ms, 0.1-10 W, 980 nm) were used to stimulate: A) in vitro: excised patches from HEK293 cells expressing TRPV1; B) in vitro: rat DRG nociceptors expressing either TRPV1 or TRPV2; and C) in vivo: C-fibers of the rat saphenous nerve (SN) trunk. Cell currents were recorded using standard patch clamp methods. The SN was also stimulated electrically with bipolar electrodes. Stimulation (20-50 ms) of HEK and DRG cells expressing TRPV1 was highly reproducible. Activation and peak currents were achieved at estimated peak temperatures of 55°C and 70°C. Threshold activation was also observed in DRG neurons expressing TRPV2. The conduction velocity for laser-activated saphenous nerve afferents was in the C fiber range (0.5-1 m/s). Electrically stimulated nerve contained stimulation artifacts and complex neural components with conduction velocities ranging from 0.3-30 m/s. Diode laser activation of TRPV1 protein is a reproducible and effective means to probe TRP activity in both in vivo and in vitro preparations

Citation Download Citation

Nan Jiang, Brian Y. Cooper, and Michael I. Nemenov "Noninvasive diode laser activation of transient receptor potential proteins and nociceptors", Proc. SPIE 6428, Mechanisms for Low-Light Therapy II, 642809 (21 February 2007); https://doi.org/10.1117/12.699204

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