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Lipid rafts association of synaptotagmin I on synaptic vesicles

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Abstract

We confirmed the raft association of synaptotagmin I (syt I) in synaptic vesicles by sucrose density gradient centrifugation, cholesterol depletion, and temperature dependence, and Ca2+ was found to positively regulate this association. Furthermore, using syt I mutants we found that the transmembrane domain (TMD) of syt I plays an important role in localizing syt I into the lipid rafts of synaptic vesicles, and the raft association of the TMD can be regulated by its phosphorylation status.

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Abbreviations

MbCD:

methyl-β-cyclodextrin

SNAP-25:

synaptosomal-associated protein of 25 kD

SNARES:

soluble N-ethylmaleimide-sensitive factor attachment protein receptors

syt I:

synaptotagmin I

TMD:

transmembrane domain of syt I

VAMP:

vesicle-associated membrane protein

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Authors and Affiliations

  1. Department of Biological Sciences and Biotechnology, State-Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing, 100084, China

    Ji-Hua Lv, Li He & Sen-Fang Sui

Authors
  1. Ji-Hua Lv
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  2. Li He
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  3. Sen-Fang Sui
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Correspondence to Sen-Fang Sui.

Additional information

Published in Russian in Biokhimiya, 2008, Vol. 73, No. 3, pp. 349–355.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM07-286, January 28, 2008.

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Lv, JH., He, L. & Sui, SF. Lipid rafts association of synaptotagmin I on synaptic vesicles. Biochemistry Moscow 73, 283–288 (2008). https://doi.org/10.1134/S0006297908030073

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  • DOI: https://doi.org/10.1134/S0006297908030073

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