Abstract
The delta-sarcoglycan-deficient hamster is an excellent model to study muscular dystrophy. Gait disturbances, important clinically, have not been described in this animal model. We applied ventral plane videography (DigiGait) to analyze gait in BIO TO-2 dystrophic and BIO F1B control hamsters walking on a transparent treadmill belt. Stride length was ~13% shorter () in TO-2 hamsters at 9 months of age compared to F1B hamsters. Hindlimb propulsion duration, an indicator of muscle strength, was shorter in 9-month-old TO-2 ( ms) compared to F1B hamsters ( ms; ). Braking duration, reflecting generation of ground reaction forces, was delayed in 9-month-old TO-2 ( ms) compared to F1B hamsters ( ms; ). Hindpaw eversion, evidence of muscle weakness, was greater in 9-month-old TO-2 than in F1B hamsters ( versus ; ). Incline and decline walking aggravated gait disturbances in TO-2 hamsters at 3 months of age. Several gait deficits were apparent in TO-2 hamsters at 1 month of age. Quantitative gait analysis demonstrates that dystrophic TO-2 hamsters recapitulate functional aspects of human muscular dystrophy. Early detection of gait abnormalities in a convenient animal model may accelerate the development of therapies for muscular dystrophy.
1. Introduction
Muscular dystrophy includes loss of muscle strength and muscle mass and leads to gait disturbances resulting in compensatory kinematic and postural adaptation [1–3]. Eventually, patients lose their ability to stand upright and walk. Clinical gait analysis of subjects with muscular dystrophy is widely used to test the efficacy of new drugs because there are few reliable methods to determine function in muscular dystrophy [1]. Histopathological studies of animal models, including the delta-sarcoglycan-deficient dystrophic hamster, have played an important role in our understanding and treatment of muscular dystrophy [4]. Functional phenotypic evidence, however, of restoration of muscle strength with therapy in animal models, is becoming increasingly important in preclinical drug development [5–9]. A recent study of canine muscular dystrophy accounted for the slower walking speed in dystrophic subjects to more accurately quantify the kinematics of pathologic gait during overground walking [10]. Treadmill gait analysis provides the opportunity to have all subjects walk at the same speed to rule out differences in walking speed as being the most important confounder in the interpretation of gait disturbances. Treadmill gait analysis has recently been applied to the characterization of mouse models of amyotrophic lateral sclerosis [11, 12], rat models of nerve injury [13, 14], and canine arthritis [15]. Quantitative kinematic gait analysis in a small animal model of muscular dystrophy, however, has not yet been reported. We, therefore, performed treadmill gait analysis in the BIO TO-2 strain of myopathic hamsters to fully characterize their posture and kinematics during walking. The BIO TO-2 [TO-2] hamster, with a deletion in the delta-sarcoglycan gene, is a widely studied animal model of muscular dystrophy [16–20]. These animals show pathological signs of muscular dystrophy, including elevated serum creatine kinase and central nucleation and necrosis of muscle fibers. We compared gait in the myopathic hamsters to the gait in healthy BIO F1B [F1B] control hamsters and found several functional indices that significantly and consistently deviate beginning early in life. The method described in this report provides quantitative functional metrics of gait associated with sarcoglycan deficiency in a readily available animal model.
2. Methods
2.1. Animals
Male BIO F1B control and BIO TO-2 dystrophic hamsters were obtained from Bio Breeders, Inc., Fitchburg, MA. Handling and care of hamsters were consistent with federal guidelines and approved institutional protocols.
2.2. Gait Dynamics
Gait dynamics were recorded using ventral plane videography, as previously described [21, 22]. Briefly, we devised a motor-driven treadmill with a transparent treadmill belt [DigiGait Imaging System, Mouse Specifics, Inc., Quincy, MA]. A high-speed digital video camera was positioned below the transparent belt to focus on the ventral view of subjects walking atop of the belt. An acrylic compartment, ~7 cm wide by ~30 cm long and adjustable for length and width, was mounted on top of the treadmill to maintain the hamsters within the view of the camera. Figure 1(a) depicts a 9-month-old TO-2 hamster walking on the treadmill belt within the acrylic compartment. Digital video images of the underside of hamsters were recorded at ~125 frames per second. Each image represents ~8 ms; the paw area indicates the temporal placement of the paw relative to the treadmill belt. The color images (Figure 1(b), top panel) were converted to their binary matrix equivalents (Figure 1(b), bottom panel) and the areas of the approaching or retreating paws relative to the belt and camera were calculated throughout each stride. The plotted area of each digital paw print (paw contact area) imaged sequentially in time provides a dynamic gait signal, representing the temporal record of paw placement relative to the treadmill belt (Figure 1(c)). Each gait signal for each limb comprises a stride duration which includes the stance duration when the paw of a limb is in contact with the walking surface, plus the swing duration when the paw of the same limb is not in contact with the walking surface. Stance duration was further subdivided into braking duration (increasing paw contact area over time) and propulsion duration (decreasing paw contact area over time) (Figure 1(c)).
Stride frequency was calculated from the number of gait signals over time. Stride length was calculated from the following equation: speed = stride frequency × stride length. Stance widths and paw placement angles at full stance were obtained by fitting ellipses to the paws and determining the centers, vertices, and major axes of the ellipses. Forelimb and hindlimb stance widths were calculated as the perpendicular distance between the major axis of the left and right fore paw images and between the major axis of the left and right hindpaw images during peak stance. Paw placement angle was calculated as the angle that the long axis of a paw makes with the direction of motion of the animal during peak stance. Gait data were collected and pooled from both the left and right forelimbs, and the left and right hindlimbs.
Measures of stride-to-stride variability (gait variability) for stride length and stance width were determined as the standard deviation and the coefficient of variation (CV). The standard deviation reflects the dispersion about the average value for a parameter. CV, expressed as a %, was calculated from the following equation: 100 × standard deviation/mean value.
Each hamster was allowed to explore the treadmill compartment for ~1 minute to acclimatize it to the apparatus. There are few data on hamsters walking on motorized treadmills. We therefore randomly selected TO-2 and F1B hamsters and subjected them to a range of walking speeds. We selected 16 cm/s for the 3-month- and 9-month-old hamsters since most animals walked comfortably at this speed and it was sufficiently fast to prevent the hamsters from rearing or turning around during videography. We selected a walking speed of 25 cm/s for the more active 1-month-old hamsters. We recorded ~3 seconds of video images for each hamster walking at a speed of 16 cm/s to provide more than 7 sequential strides. Only video segments of hamsters walking with a regularity index of 100% [23] were used for image analysis. For incline and decline walking, the treadmill, walking compartment, and camera system were pitched at an angle so that the animals walked up an incline of ~15° or down a decline of ~15°, dependent on the direction of the treadmill belt. The angle of treadmill incline and decline was based on published protocols [8, 16, 24]. The treadmill belt was wiped clean between studies if necessary.
2.3. Statistics
Data are presented as means ± SE. ANOVA was used to test for statistical differences among TO-2 and F1B hamsters at each age. When the -score exceeded for , we used post-hoc unpaired Student's two-tailed -tests to compare group means. Gait indices between forelimbs and hindlimbs within groups were compared using paired Student's two-tailed -tests. Differences were considered significant with .
3. Results
3.1. Gait Dynamics in Hamsters
The ventral view of a 9-month-old healthy F1B control hamster walking on a transparent treadmill belt is shown in Video 1 (Video 1, available as supplemental material). Gait indices for adult F1B hamsters are summarized in Table 1. F1B hamsters () walked ~2 steps every second, completed one stride within ~500 ms, and traversed ~8 cm with each step walking at a speed of 16 cm/s. The contributions of stance and swing duration to stride duration were ~75% (stance/stride) and ~25% (swing/stride).
Differences in the posture and gait dynamics of the forelimbs and hindlimbs were quantified. Forelimb stance width was significantly narrower than hindlimb stance width ( cm versus cm, ). Stance width variability (CV%) of hindlimbs was lower than that of forelimbs in 9-month-old F1B hamsters walking at a speed of 16 cm/s. Figure 2 illustrates the relative contributions of swing, stance, braking, and propulsion to the stride duration of forelimbs and hindlimbs in 9-month-old F1B hamsters. Swing duration of the forelimbs was slightly longer than of the hindlimbs. The braking phase was greater in forelimbs than in hindlimbs (% versus %, ), and the propulsion phase was greater in hindlimbs than in forelimbs (% versus %, ) with comparable stance durations of forelimbs ( ms) and hindlimbs ( ms).
3.2. Gait in Dystrophic Hamsters
Gait dynamics were significantly different in 9-month-old TO-2 dystrophic hamsters () compared to age-matched F1B control hamsters () (Table 1). Figure 3(a) illustrates the gait signal from the left hindlimb of a TO-2 hamster superimposed over the gait signal of the left hindlimb of a F1B hamster. Stride frequency was increased in TO-2 compared to F1B hamsters. Stride duration was significantly shorter in TO-2 than that in F1B hamsters ( ms versus ms; ). This was attributable to a shorter swing duration ( versus ms; ), and a shorter stance duration ( ms versus ms; ) in TO-2 compared to F1B hamsters. Stride length was decreased in TO-2 compared to F1B hamsters ( cm versus cm; ) at a walking speed of 16 cm/s. Forelimb stance width was narrower in TO-2 compared to F1B hamsters ( cm versus cm; ). The paw placement angle of the hindlimbs was also more significantly everted in TO-2 than in F1B hamsters ( versus ; ). Forelimb stride length variability was higher in TO-2 than in F1B hamsters (% versus %; ). The braking duration of the forelimbs, which is the time needed to decelerate the animal, was prolonged in TO-2 compared to F1B hamsters ( ms versus ms; ). The propulsion duration of the hindlimbs, which reflects the interval available for force generation, was shortened in TO-2 compared to F1B hamsters ( ms versus ms; ) (Figure 3(a), arrows). Figure 3(b) illustrates the relative contributions of braking and propulsion to the stance duration of forelimbs and hindlimbs in 9-month-old F1B control hamsters and TO-2 delta-sarcoglycan-deficient hamsters walking at 16 cm/sec. The relative contributions of forelimb braking to stance were significantly altered in TO-2 dystrophic hamsters.
(a)
(b)
3.3. Early Functional Phenotype of Muscular Dystrophy
Most of the gait disturbances observed in 9-month-old dystrophic hamsters were also apparent at 3 months of age. Stride length was decreased in 3-month-old TO-2 dystrophic hamsters () compared to F1B control hamsters () ( cm versus cm; ) at a walking speed of 16 cm/s. Hindlimb stance width was narrower in TO-2 than in F1B hamsters ( cm versus cm; ). Forelimb stance width was significantly narrower in TO-2 than in F1B hamsters when the animals were challenged to walk uphill or downhill (Figure 4). As in older animals, the paw placement angle of the 3-month-old dystrophic TO-2 hindlimbs was more significantly everted than that in F1B controls ( versus ; ). Whereas the braking duration of the forelimbs was not different in 3-month-old TO-2 from F1B hamsters walking horizontally, the propulsion duration of the hindlimbs was shortened in 3-month-old TO-2 hamsters ( ms versus ms; ). Challenging the animals to walk uphill and downhill, moreover, exacerbated functional differences between 3-month-old TO-2 () and F1B hamsters () (Figure 4).
Even at 1 month of age, several gait indices deviated significantly in TO-2 dystrophic hamsters. Compared to 1-month-old F1B control hamsters (), 1-month-old TO-2 dystrophic hamsters () were characterized by significantly shorter stride length ( cm versus cm), more eversion of the hindpaw ( versus ), shortened hindlimb propulsion duration ( ms versus ms), and prolonged forelimb braking duration ( ms versus ms) (all ). Forelimb stride length variability tended to be higher in 1-month-old TO-2 compared to F1B hamsters (% versus %; ). Figure 5 illustrates differences in stride length between F1B control and TO-2 dystrophic hamsters across the three ages studied.
4. Discussion
This is the first comprehensive analysis of posture and gait in hamsters, and the first quantitative analysis of gait changes in a small animal model of muscular dystrophy. Gait analysis of patients with muscular dystrophy is implemented to assess disease progression [1–3]. Mouse and hamster models are widely used to better understand and develop therapies for muscular dystrophy; yet, very little is known about how these animals walk. The postural and kinematic gait data we provide in F1B control hamsters and delta-sarcoglycan-deficient TO-2 dystrophic hamsters may help to establish reference values for future studies aimed at reversing gait abnormalities with potential therapeutics tested in this animal model.
There are at least nine different types of muscular dystrophy. Loss of muscle strength is common to all types of muscular dystrophies; age of onset, muscle groups involved, and progression vary. Mutations in the sarcoglycan-gene result in limb-girdle muscular dystrophy (LGMD), of which there are different types [25]. The limb-girdle (shoulders and hips) muscles are usually the first affected, with muscle weakness becoming more progressive and spreading to more distal muscles, including tibialis anterior [26], wrists [27], and hands [28]. Delta-sarcoglycan deficiency results in LGMD-type 2F [29]. Most LGMD-type 2F patients have severe muscular dystrophy that presents early in life and progresses rapidly.
The TO-2 hamster, with a deletion in the delta-sarcoglycan gene and deficiency of alpha-, beta-, and gamma-sarcoglycan, has been widely studied [16–20] and is considered a good animal model of human LGMD-type 2F. Gross examination upon autopsy of the parental strain of TO-2 hamsters indicated that shoulder muscles were the most affected [30]. Pathology has also indicated damage to the intercostal muscles, proximal limb muscles, muscles of the pelvic girdle, and muscles of the back and the abdominal wall [17]. Gastrocnemius, triceps, and tibialis muscles have also been shown to be affected [7, 16, 20, 31]. All of these muscles are involved in quadrupedal gait.
Despite extensive pathology evidence of muscle damage, very little is known about muscle dysfunction in animal models of muscular dystrophy, including the TO-2 hamster [32]. Xiao et al. demonstrated reduced tibialis anterior muscle strength in dystrophic hamsters [20]. Zhu et al. reported reduced treadmill running endurance as a measure of weakness in TO-2 hamsters [16]. Vitiello et al. concluded that gene therapy ameliorated muscular dystrophy in hamsters based on their improved rotarod performance [7]. Our treadmill gait analysis study sought to reconcile systemic muscle weakness with the ability to walk, functionality most important to patients with muscular dystrophy.
Observation of TO-2 hamsters in their cage does not suggest that they have gait disturbances, which is why we studied treadmill gait analysis in 9-month-old animals, an age of which the animals are known to have significant muscle weakness. The ~13% shorter stride length in the dystrophic hamsters is a very robust deficit; in otherwise healthy patients with neuromuscular disease, ~10% reductions in stride length can be clinically relevant. Because the treadmill speed was set to 16 cm/s for both TO-2 dystrophic and F1B control animals, the shorter stride length in the TO-2 animals required significantly increased stepping frequency. Increased stepping frequency as part of daily life poses increased cardiorespiratory demands [33]. The paw placement angle of the hindlimbs was more open in TO-2 animals, suggesting weakness of muscle in the hindpaws or lower hindlimbs. Though the hindlimb propulsion duration was shorter, the percentage of contribution of the hindlimbs to propulsion was not different in TO-2 from that of F1B hamsters, suggesting that the shorter propulsion duration was secondary to a shorter stride cycle.
There were several important differences in forelimb gait in TO-2 dystrophic compared to F1B control hamsters. This is in contrast to some animal models of neuromuscular diseases, such as the SOD1 G93A mouse model of amyotrophic lateral sclerosis, in which the gait disturbances are more localized to the hindlimbs [11]. The forelimb stance width was significantly narrower; often, changes in base of support are compensatory to muscular and vestibular defects to maintain posture and balance. The forelimb braking duration was prolonged, but more significantly, the percentage contribution of forelimb deceleration was larger in TO-2 hamsters. Taken together, muscle weakness of the shoulder girdle and forelimbs might contribute substantively to the gait disturbances in TO-2 hamsters. This shoulder girdle functional readout is notable given that the supraspinatus muscle was the most severely affected in autopsied dystrophic hamsters [30], the relatively larger load carried by the upper limbs in quadrupeds compared to humans, and the known involvement of the shoulder girdle in LGMD patients.
The differences in gait between 3-month-old F1B and TO-2 hamsters were strikingly similar to those observed in older animals. This may not be surprising, however, since histopathology indicates extensive muscle pathology at this age [16, 17, 19, 31, 34]. Challenging the animals to incline or decline walking exacerbated postural and kinematic differences. Downhill walking especially increased the magnitude of differences in stride length, stance width, and propulsion duration. Walking up an incline or down a decline is known to changes muscle load and force distribution [35, 36]; this is especially true for quadrupeds that use their forelimbs for weight support. Downhill walking has been shown to increase loading on the shoulders and upper limbs of pongids [37], cats [36], and rats [38]. Shoulder muscles are particularly vulnerable in LGMD. The observations of exacerbated upper limb gait disturbances in the TO-2 hamsters downhill walking suggest that the shoulder girdle may be more affected, at least functionally, than the hip girdle in the delta-sarcoglycan-deficient hamster model of muscular dystrophy.
Skeletal muscle pathology is already evident in TO-2 animals one month old [17, 19, 30, 34]. We observed significant differences in posture and gait between 1-month-old TO-2 dystrophic and F1B control hamsters. Several metrics, including shorter stride length and greater hindpaw eversion, were consistent findings in juvenile (1-month-old), young (3-month-old), and adult (9-month-old) delta-sarcoglycan-deficient dystrophic hamsters. In summary, gait analysis in delta-sarcoglycan-deficient dystrophic BIO TO-2 hamsters provides early functional evidence of systemic muscle weakness that could accelerate the screening of potential therapies for muscular dystrophy.
Disclosure
T. G. Hampton is owner of Mouse Specifics, Inc., a company that has commercialized the gait analysis instrumentation. C. G. VanDongen. is owner of BioBreeders, Inc., a purveyor of hamsters.
Acknowledgments
This paper is dedicated to the memory of Dr. Ivo Amende, a clinician and scholar who readily translated animal research to the clinics, and taught the authors much about human nature. Cecelia Hampton is acknowledged for her helpful comments on the manuscript.
Supplementary Materials
This video describes “Gait Dynamics in Hamsters” and shows the ventral view of a 9-month-old healthy F1B control hamster walking on a transparent treadmill belt.