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Licensed Unlicensed Requires Authentication Published by De Gruyter October 16, 2009

Non-radioactive Labeling and Detection of Nucleic Acids. II. Optimization of the Digoxigenin System

  • Hans-Joachim HÖLTKE , Rudolf SEIBL , Josef BURG , Klaus MÜHLEGGER and Christoph KESSLER
From the journal Biological Chemistry
https://doi.org/10.1515/bchm3.1990.371.2.929
Published Online: 2009-10-16
Published in Print: 1990

Walter de Gruyter

From the journal
Biological Chemistry
Biological Chemistry
Volume 371 Issue 2
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Journal and Issue

Articles in the same Issue

Titelei
The Complete Amino-Acid Sequence and the Phylogenetic Origin of Phycocyanin-645 from the Cryptophytan alga Chroomonas sp.
Monoclonal Antibodies Specific for Neutrophil Proteinase 4. Production and Use for Isolation of the Enyzme
Hemocyanins in Spiders, XXIII. Complete Amino-Acid Sequence of Subunit a of Eurypelma californicum hemocyanin
Brefeldin A Prevents Uncovering but not Phosphorylation of the Recognition Marker in Cathepsin D
Different Forms of Human Cystatin C
Studies on the Extraction of Different Proteoglycan Populations in Bovine Articular Cartilage
Inhibition of Serine Proteinases by Squash Inhibitors
Purification and N-Terminal Amino-Acid Sequence Analysis of Rat Polymorphonuclear Leukocyte Cathepsin G
Purification and Characterization of Bovine Liver. 3-cis-2-trans-Enoyl-CoA Isomerase
Separation of Granule Subpopulations in Human Polymorphonuclear Leukocytes
Detection of Cathepsin B, Plasminogen Activators and Plasminogen Activator Inhibitor in Human Non-Small Lung Cancer Cell Lines
Subcellular Distribution of Adenine Nucleotides in Two Ehrlich Cell Lines Metabolizing Glucose
Nucleotide Sequences of Bacillus stearothermophilus Ribosomal Protein Genes: Part of the Ribosomal S10 Operon
Einfluß von Nicotinamidgabe auf das Gallensäuremuster bei Streptozotocin-behandelten Ratten
Primary Structure and Oxygen-Binding Properties of the Hemoglobin from Guanaco (Lama guanacoë, Tylopoda)
The Complete Primary Structure of α-Lactalbumin Isolated from Pig (Sus scrofa) Milk
Structure and Function of L-Lactate Dehydrogenases from Thermophilic and Mesophilic Bacteria, X. Analysis of Structural Elements Responsible for the Differences in Thermostability and Activation by Fructose 1,6-bisphosphate in the Lactate. Dehydrogenases from B. stearothermophilus and B. caldolyticus by Protein Engineering
Partial Purification and Properties of Cathepsin G-like Proteinase of Mouse Myeloid Leukemia M1 Cells
Cooperativity and Intermediate States in the T→R-Structural Transformation of Insulin
A Facile Method for the Isolation and Preparation of Proteins and Peptides for Sequence Analysis in the Picomolar Range
Protective Role of Transforming Growth Factor β (TGF β) in Tumor-Induced Degradation of Basement Membranes
Effect of Nonenzymatic Glycation on the Structure of Immunoglobulin G
Dipeptidyl Peptidase IV in the Immune System. Cytofluorometric Evidence for Induction of the Enzyme on Activated T Lymphocytes
On the Action of Carboxy Groups in the Citrate Synthase Reaction
4-Methylumbelliferyl-α-Glycosides of Partially 0-Acetylated N-Acetylneuraminic Acids as Substrates of Bacterial and Viral Sialidases
Activation of the Human Leukocyte Proteinases Elastase and Cathepsin G by Various Surfactants
Selective Inhibition of Phospholipase A2 by Different Lipocortins
Addendum to Partial Amino-Acid Sequence of Human PMN Leukocyte Procollagenase
85. Konferenz der Gesellschaft für Biologische Chemie. Studiengruppe „Biochemische Pharmakologie und Toxikologie“. Proteinkinase C and Calcium
86. Konferenz der Gesellschaft für Biologische Chemie. Studiengruppe „Protein- und Nucleinsäure-Analytik“. Modern Methods in Protein and Nucleic Acid Analysis
4th Joint Meeting. Société de Chimie Biologique Schweizerische Gesellschaft für Biochemie Gesellschaft für Biologische Chemie
Isolation and Partial Characterization of Cyclosporin Synthetase from a Cyclosporin Non-Producing Mutant of Beauveria nivea
Fluorogenic Substrates for Chymotrypsin with 2-Quinolinone Derivatives as Leaving Groups
Regulation of the Fructose 6-phosphate/Fructose 2,6-bisphosphate Cycle by Enzyme Phosphorylation and sn-Glycerol 3-phosphate
The Midgut Trypsins of Shrimp (Penaeus monodon). High Efficiency Toward Native Protein Substrates Including Collagens
Synthesis of a Water-Soluble Protein Cross-Linking Reagent
Crossed Immunoelectrophoresis Does not Allow Accurate Determination of Inter-α-Trypsin Inhibitor and Its Derivatives in Plasma
Covalent Structure of the Minor Monomeric β-Lactoglobulin II Component from Donkey Milk
On the Mechanism of Hydrogen Peroxide Formation during Precipitation of Hemoglobin with Perchloric Acid
Serine Proteinase from Cucurbita ficifolia Seed; Purification, Properties, Substrate Specificity and Action on Native Squash Trypsin Inhibitor (CMTI I)
83. Konferenz der Gesellschaft für Biologische Chemie. Molecular Mechanisms of Sensory Transduction in Bacterial and Animal Cells
Polyol Determination along the Rat Nephron
Non-radioactive Labeling and Detection of Nucleic Acids. I. A Novel DNA Labeling and Detection System Based on Digoxigenin: Anti-Digoxigenin ELISA Principle (Digoxigenin System)
Non-radioactive Labeling and Detection of Nucleic Acids. II. Optimization of the Digoxigenin System
Non-radioactive Labeling and Detection of Nucleic Acids. III Applications of the Digoxigenin System
Non-radioactive Labeling and Detection of Nucleic Acids. IV. Synthesis and Properties of Digoxigenin-modified 2´ -Deoxyuridine-5’ -triphosphates and a Photoactivatable Analog of Digoxigenin (Photodigoxigenin)
Conjugates of Synthetic Lymphocyte-Activating Lipopeptides with Segments from HIV Proteins Induce Protein-Specific Antibody Formation
Ile-Ser-Bradykinin is an Aberrant Permeability Factor in Various Human Malignant Effusions
Modulation by Oxygen of the Actions of Noradrenaline, Sympathetic Nerve Stimulation and Prostaglandin F2α on Carbohydrate Metabolism and Hemodynamics in Perfused Rat Liver
Human Urinary Trypsin Inhibitor (Urinastatin)-Like Substance in Mouse Kidney and its Relationships to Mouse Kidney Kallikrein
Microbial Metabolism of Quinoline and Related Compounds. V. Degradation of 1H-4-Oxoquinoline by Pseudomonas putida 33/1
Microbial Metabolism of Quinoline and Related Compounds. VI. Degradation of Quinaldine by Arthrobacter sp.
Comparative Analytical Studies on Recombinant and Native Human Somatrotropin
Carnivora: The Primary Structure of Hemoglobin from the Masked Palm Civet (Paguma larvata, Viverridae)
Human Fibroblasts Release Low Amounts of Reactive Oxygen Species in Response to the Potent Phagocyte Stimulants, Serum-Treated Zymosan, N-Formyl-methionyl-leucyl-phenylalanine, Leukotriene B4 or 12-O-Tetradecanoylphorbol 13-Acetate
88. Konferenz der Gesellschaft für Biologische Chemie. Biochemie des Nervensystems. Herbsttagung der „Studiengruppe Neurochemie“
Studies on Human Porin. III. Does the Voltage-Dependent Anion Channel “Porin 31HL” Form Part of the Chloride Channel Complex, which is Observed in Different Cells and Thought to be Affected in Cystic Fibrosis?
Labeling of Precursor Pools for Glycosphingolipid Biosynthesis. Incorporation of [3H]Galactose by Rat Hepatocytes in Primary Culture
Studios on the Total Synthesis of an A7,B7-Dicarbainsulin. III. Assembly of Segments and Generation of Biological Activity
Comparison of the Effects of Fibrinogen and Fibrin Products and Isolated Peptide Chains on the Fibrin-Mediated Stimulation of Plasminogen Activation by Tissue-Type Plasminogen Activator, and on the Fibrin-Dependent Enhancement of the Amidolytic Activity of One-Chain Tissue-Type Plasminogen Activator
Properties of 5-Hydroxyvalerate CoA-Transferase from Clostridium aminovalericum
The Preparation of Fully Active Chymopapain Free of Contaminating Proteinases
The Primary Structures of Gundi (Ctenodactylus gundi, Rodentia). Hemoglobin and Myoglobin
High Production of Polysialic Acid [Neu5Acα(2-8)-Neu5Acα(2-9)]n by Escherichia coli K92 Grown in a Chemically Defined Medium. Regulation by Temperature
High Affinity Binding of Immunoglobulin G Fragments to Human Polymorphonuclear Leukocytes
The Degradation of Bioactive Peptides and Proteins by Dipeptidyl Peptidase IV from Human Placenta
Isolation of Plasminogen Activator Inhibitor-2 (PAI-2) from Human Placenta. Evidence for Vltronectin/PAI-2 Complexes in Human Placenta Extract
The Heterotopic Effects of Insulin and Glucagon on the Acinar Activity Pattern of Phosphoenolpyruvate Carboxykinase in Male and Female Rat Liver
Microbial Metabolism of Quinoline and Related Compounds. VII. Quinoline Oxidoreductase from Pseudomonas putida: a Molybdenum-Containing Enzyme
Probing of Active Site Residues of the Zinc Enzyme 5-Aminolevulinate Dehydratase by Spin and Fluorescence Labels
Inter-α-Trypsin Inhibitor and Pre-α-Trypsin Inhibitor in Health and Disease. Determination by Immunoelectrophoresis and Immunoblotting
Purification and Characterization of Prolyl Endopeptidase from Pig Brain
The Self-Association of Zinc-Free Bovine Insulin. Four Model Patterns and Their Significance
A Point Mutation at the X-Chromosomal Proteolipid Protein Locus in Pelizaeus-Merzbacher Disease Leads to Disruption of Myelinogenesis
Structure of the Human α1-Microglobulin-Bikunin Gene
Index of Authors
The editors and the publisher wish to express their sincere thanks for the kind assistance of the following scientists who acted as reviewers for our journal:
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