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Multiple image alignment of neuronal staining using anti-NeuN-DAB staining of sham mice and 28 days after 30 min MCAo and delayed treatment with 5 µg TAT.ARC or control protein.
dataset
posted on 2016-08-04, 08:57 authored by stefan donathstefan donath, Junfeng AnJunfeng An, Karen GertzKaren Gertz, Anna Lena DätwylerAnna Lena Dätwyler, Ulrike HarmsUlrike Harms, Susanne MuellerSusanne Mueller, Tracy FarrTracy Farr, Gisela Lättig-TünnemannGisela Lättig-Tünnemann, Janet LipsJanet Lips, Marco FoddisMarco Foddis, Larissa MoschLarissa Mosch, Rene BernardRene Bernard, Ulrike GrittnerUlrike Grittner, Mustafa BalkayaMustafa Balkaya, Golo KronenbergGolo Kronenberg, Ulrich DirnaglUlrich Dirnagl, Matthias EndresMatthias Endres, Christoph HarmsChristoph HarmsMultiple image
alignment of neuronal stainings using anti-NeuN-DAB of sham mice and 28 days
after 30 min MCAo. Mouse brains were
cut in coronal sections using a mouse brain atlas (Franklin and Paxinos, 2007) to analyze direct and indirect infarct volumes, striatal area in a3
(area 3) and neuronal densities within the striatum in a3: a1: Bregma = 2.80;
a2: Bregma = 1.54; a3: Bregma = 0.14; a4: Bregma = -1.94; a5: Bregma =
-3.88. Image collection of
NeuN-DAB-stained brain slices from mice 28 days post MCAo were collected as
transmission images using a Leica DMI8 microscope equipped with LED light
source, a Fluotar 10x/030 dry objective and a DFC300G camera and stitched
within the Leica LAS X2.0 software. The code of mice ID can be found in the excel spread sheet attached. a1-a5 and mouse ID is used in the file name.
