Amplification of cDNA ends based on template-switching effect and step-out PCR

M Matz; D Shagin; E Bogdanova; O Britanova; S Lukyanov; L Diatchenko; A Chenchik

doi:10.1093/nar/27.6.1558

Amplification of cDNA ends based on template-switching effect and step-out PCR

Nucleic Acids Res. 1999 Mar 15;27(6):1558-60. doi: 10.1093/nar/27.6.1558.

Authors

M Matz¹, D Shagin, E Bogdanova, O Britanova, S Lukyanov, L Diatchenko, A Chenchik

Affiliation

¹ Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow 117871, Russia.

Abstract

A new method for amplifying cDNA ends is described which requires only first-strand cDNA synthesis and a single PCR to generate a correct product with very low or no background. The method can be successfully applied to total RNA as well as poly A+ RNA. The same first-strand cDNA can be used to amplify flanking sequences of any cDNA species present in the sample.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA, Complementary / genetics*
Models, Genetic
Polymerase Chain Reaction / methods*

Substances

DNA, Complementary