Abstract
Synaptic scaffolding molecule (S-SCAM) is a multiple PDZ domain-containing protein, which interacts with neuroligin, a cell adhesion molecule, and the NMDA receptor. In this study, we searched for S-SCAM-interacting proteins and obtained a neuralplakophilin-related armadillo-repeat protein (NPRAP)/delta-catenin. NPRAP/delta-catenin bound to the last PDZ domain of S-SCAM via its carboxyl-terminus in three different cell-free assay systems, was coimmunoprecipitated with S-SCAM from rat crude synaptosomes, and was localized at the excitatory synapses in rat hippocampal neurons. NPRAP/delta-catenin may be implicated in the molecular organization of synaptic junctions through the interaction with S-SCAM.
Copyright 1999 Academic Press.
MeSH terms
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Adaptor Proteins, Signal Transducing
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Animals
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Armadillo Domain Proteins
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Binding Sites
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Biosensing Techniques
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COS Cells
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Carrier Proteins / chemistry
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Carrier Proteins / genetics
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Carrier Proteins / metabolism*
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Gene Library
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Guanylate Kinases
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Hippocampus / cytology
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Hippocampus / metabolism
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Kinetics
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Molecular Weight
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Nerve Tissue Proteins / chemistry
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / metabolism*
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Neurons / metabolism*
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Peptide Fragments / chemical synthesis
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Peptide Fragments / metabolism
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Precipitin Tests
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Protein Binding
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Rats
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Receptors, N-Methyl-D-Aspartate / metabolism
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Synaptosomes / metabolism
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Transfection
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Yeasts / genetics
Substances
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Adaptor Proteins, Signal Transducing
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Armadillo Domain Proteins
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Carrier Proteins
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Ctnnd2 protein, rat
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Magi2 protein, rat
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Nerve Tissue Proteins
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Peptide Fragments
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Receptors, N-Methyl-D-Aspartate
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Recombinant Fusion Proteins
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Guanylate Kinases