The pest regions containing C-termini of mammalian ornithine decarboxylase and histidine decarboxylase play different roles in protein degradation

Biochem Biophys Res Commun. 1999 Apr 13;257(2):269-72. doi: 10.1006/bbrc.1999.0456.

Abstract

Proteasome 26S must recognize the PEST region-containing C-terminus of mammalian ornithine decarboxylase (ODC) monomer to proceed with degradation. We have detected PEST regions in both termini of mammalian histidine decarboxylase (HDC). In the present report, a chimaeric ODC/HDC was used to elucidate whether the PEST region-containing C-termini of ODC and HDC are exchangeable. Wild-type rat ODC had an expected antizyme and ATP-dependent degradation. This was not the case for both the chimaera and a C-terminus truncated rat ODC. Results suggest that the PEST region-containing C-terminus of rat HDC should have another role different to confering polypeptide availability to the proteasome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / metabolism
  • Amino Acid Sequence
  • Animals
  • Enzyme Stability
  • Half-Life
  • Histidine Decarboxylase / chemistry
  • Histidine Decarboxylase / genetics
  • Histidine Decarboxylase / metabolism*
  • Kinetics
  • Ornithine Decarboxylase / chemistry
  • Ornithine Decarboxylase / genetics
  • Ornithine Decarboxylase / metabolism*
  • Peptide Hydrolases / metabolism
  • Proteasome Endopeptidase Complex*
  • Protein Biosynthesis
  • Protein Conformation
  • Rats
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Sequence Deletion
  • Structure-Activity Relationship
  • Transcription, Genetic

Substances

  • Recombinant Fusion Proteins
  • Adenosine Triphosphate
  • Peptide Hydrolases
  • Proteasome Endopeptidase Complex
  • ATP dependent 26S protease
  • Ornithine Decarboxylase
  • Histidine Decarboxylase