The amino-terminus of the amyloid-beta protein is critical for the cellular binding and consequent activation of the respiratory burst of human macrophages

F L Van Muiswinkel; S F Raupp; N M de Vos; H A Smits; J Verhoef; P Eikelenboom; H S Nottet

doi:10.1016/s0165-5728(99)00019-3

The amino-terminus of the amyloid-beta protein is critical for the cellular binding and consequent activation of the respiratory burst of human macrophages

J Neuroimmunol. 1999 Apr 1;96(1):121-30. doi: 10.1016/s0165-5728(99)00019-3.

Authors

F L Van Muiswinkel¹, S F Raupp, N M de Vos, H A Smits, J Verhoef, P Eikelenboom, H S Nottet

Affiliation

¹ Graduate School Neurosciences Amsterdam, Research Institute Neurosciences, Vrije Universiteit, Faculty of Medicine, Department of Pharmacology, The Netherlands. flv.muiswinkel.pharm@med.vu.nl

PMID: 10227431
DOI: 10.1016/s0165-5728(99)00019-3

Abstract

Here, we show that amyloid-beta (Abeta) is capable to prime and activate the respiratory burst of human macrophages. Previously, the N-terminus of Abeta(1-42) has been shown to contain a cell binding domain that is implicated in eliciting neuropathogenic microglia in vitro. To evaluate the role of this domain in the Abeta(1-42)-induced respiratory burst activity, the effect of Abeta subfragments on the Abeta(1-42)-induced superoxide release were studied. On the basis of the antagonistic properties of Abeta(1-16), it is concluded that the N-terminal region of Abeta is critical for the cellular binding and consequent activation of the respiratory burst of human phagocytes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alzheimer Disease / immunology
Alzheimer Disease / metabolism
Amyloid beta-Peptides / chemistry*
Amyloid beta-Peptides / metabolism
Amyloid beta-Peptides / pharmacology*
Binding, Competitive / drug effects
Binding, Competitive / immunology
Brain Chemistry / immunology
Flow Cytometry
Fluorescein-5-isothiocyanate
Fluorescent Dyes
Humans
Luminescent Measurements
Macrophages / chemistry
Macrophages / immunology*
Macrophages / metabolism*
Monocytes / chemistry
Monocytes / immunology
Monocytes / metabolism
Peptide Fragments / chemistry*
Peptide Fragments / metabolism
Peptide Fragments / pharmacology*
Protein Binding / immunology
Respiratory Burst / drug effects
Respiratory Burst / immunology*
Superoxides / metabolism

Substances

Amyloid beta-Peptides
Fluorescent Dyes
Peptide Fragments
amyloid beta-protein (1-11)
amyloid beta-protein (1-42)
amyloid beta-protein (25-35)
Superoxides
Fluorescein-5-isothiocyanate