Abstract
We have investigated the functional expression of the beta-chemokine receptors CCR1 to 5 in cultured rat microglia. RT-PCR analysis revealed constitutive expression of CCR1, CCR2 and CCR5 mRNA. The beta-chemokines MCP-1 (1-30 nM) as well as RANTES and MIP-1alpha (100-1000 nM) evoked calcium transients in control and LPS-treated microglia. Whereas, the response to MCP-1 was dependent on extracellular calcium the response to RANTES was not. The effect of MCP-1 but not that of RANTES was inhibited by the calcium-induced calcium release inhibitor ryanodine. Calcium responses to MCP-1- and RANTES were observed in distinct populations of microglia.
MeSH terms
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Animals
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Calcium / metabolism
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Calcium Signaling / immunology
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Cells, Cultured
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Chemokine CCL2 / pharmacology
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Chemokine CCL3
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Chemokine CCL4
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Chemokine CCL5 / pharmacology
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Cytosol / metabolism
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DNA Primers
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Enzyme Inhibitors / pharmacology
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Gene Expression / drug effects
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Gene Expression / immunology
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Lipopolysaccharides / pharmacology
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Macrophage Inflammatory Proteins / pharmacology
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Microglia / chemistry*
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Microglia / cytology
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Microglia / immunology*
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RNA, Messenger / analysis
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Rats
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Rats, Sprague-Dawley
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Receptors, CCR1
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Receptors, CCR2
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Receptors, CCR5 / genetics
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Receptors, CCR5 / immunology
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Receptors, Chemokine / genetics*
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Receptors, Chemokine / immunology*
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Recombinant Proteins / pharmacology
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Reverse Transcriptase Polymerase Chain Reaction
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Ryanodine / pharmacology
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Thapsigargin / pharmacology
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Virulence Factors, Bordetella / pharmacology
Substances
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Ccr1 protein, rat
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Ccr2 protein, rat
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Chemokine CCL2
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Chemokine CCL3
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Chemokine CCL4
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Chemokine CCL5
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DNA Primers
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Enzyme Inhibitors
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Lipopolysaccharides
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Macrophage Inflammatory Proteins
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RNA, Messenger
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Receptors, CCR1
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Receptors, CCR2
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Receptors, CCR5
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Receptors, Chemokine
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Recombinant Proteins
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Virulence Factors, Bordetella
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Ryanodine
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Thapsigargin
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Calcium