The use of anabolic agents is illegal in the European Community but the effect of these agents on animal welfare is not well documented. The aim of this study was to evaluate whether the administration of anabolic agents, such as clenbuterol, causes stressful effects in rats, as reflected by the adrenal function. Anabolic doses of clenbuterol (1 mg kg-1, 99% purity) were administered orally by stomach tube daily for 45 d to female Long Evans rats (250-300 g, n = 50). Twenty-five animals were used as controls. Blood samples were collected from the jugular vein in anaesthetised animals (ketamine and xylazine). At the end of the experiment, the animals were sacrificed and the adrenal glands were removed. Hormonal levels were measured by an enzyme immunoassay previously validated for this species. Hormonal levels of cortisol and corticosterone, and histopathological analysis, were used as indicators of the adrenal function. Increased corticosterone and cortisol secretion was found in the treated group (p < 0.001), both in adrenal homogenates and peripheral blood samples, compared with control animals. Higher relative adrenal gland weight (adrenal gland-to-body weight ratio) was also found in the treated group (p < 0.01). The major histopathological finding was the presence of hyperplasia in the adrenocortical cells. It was concluded that the administration of an anabolic dose of clenbuterol causes a hyperstimulation of adrenal gland secretion that could adversely affect animal welfare.