Clustering of the group III metabotropic glutamate receptor 4a at parallel fiber synaptic terminals in the rat cerebellar molecular layer

J M Mateos; I Elezgarai; R Benítez; A Osorio; A Bilbao; J J Azkue; R Kuhn; T Knöpfel; P Grandes

doi:10.1016/s0168-0102(99)00066-8

Clustering of the group III metabotropic glutamate receptor 4a at parallel fiber synaptic terminals in the rat cerebellar molecular layer

Neurosci Res. 1999 Oct;35(1):71-4. doi: 10.1016/s0168-0102(99)00066-8.

Authors

J M Mateos¹, I Elezgarai, R Benítez, A Osorio, A Bilbao, J J Azkue, R Kuhn, T Knöpfel, P Grandes

Affiliation

¹ Department of Neurosciences, Faculty of Medicine and Dentistry, Basque Country University, Bilbao, Spain.

PMID: 10555166
DOI: 10.1016/s0168-0102(99)00066-8

Abstract

We report in this study with a pre-embedding immunogold method, the clustering of the group III metabotropic glutamate receptor 4a (mGluR4a) along the presynaptic membrane of parallel fiber synaptic terminals in the cerebellar molecular layer. The mGluR4a clusters were homogeneously distributed and interspaced by about 60 nm. These results suggest a particular arrangement of mGluR4a which might help to a rapid and effective activation of this receptor by glutamate.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Antibodies
Cell Line
Cerebellum / ultrastructure*
Humans
Microscopy, Immunoelectron
Molecular Sequence Data
Neurons / ultrastructure*
Peptide Fragments / chemistry
Peptide Fragments / immunology
Presynaptic Terminals / ultrastructure*
Rats
Rats, Sprague-Dawley
Receptors, Metabotropic Glutamate / analysis*
Receptors, Metabotropic Glutamate / immunology
Recombinant Proteins / analysis
Synaptic Membranes / ultrastructure
Transfection

Substances

2-amino-4-phosphonobutyrate receptor
Antibodies
Peptide Fragments
Receptors, Metabotropic Glutamate
Recombinant Proteins