Density modification for macromolecular phase improvement

Prog Biophys Mol Biol. 1999;72(3):245-70. doi: 10.1016/s0079-6107(99)00008-5.

Abstract

Density modification provides a simple and largely automatic tool for improving phase estimates for observed structure factors. The phase information arises from a combination of the known structure factor magnitudes, the current phase estimates, and stereochemical information. The magnitudes, the current phase estimates, and stereochemical information. The addition of these phase information derived from theoretical sources renders new structures amenable to solution, and reduces the effort required to solve other structures. A diverse array of techniques which have been applied to the phase improvement problem are reviewed.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Algorithms
  • Crystallography, X-Ray
  • Electrons
  • Macromolecular Substances
  • Mathematics
  • Proteins / chemistry*
  • Solvents

Substances

  • Macromolecular Substances
  • Proteins
  • Solvents