Abstract
Two-photon imaging of large neuronal networks in cortical slices of newborn rats revealed synchronized oscillations in intracellular Ca2+ concentration. These spontaneous Ca2+ waves usually started in the posterior cortex and propagated slowly (2.1 mm per second) toward its anterior end. Ca2+ waves were associated with field-potential changes and required activation of AMPA and NMDA receptors. Although GABAA receptors were not involved in wave initiation, the developmental transition of GABAergic transmission from depolarizing to hyperpolarizing (around postnatal day 7) stopped the oscillatory activity. Thus we identified a type of large-scale Ca2+ wave that may regulate long-distance wiring in the immature cortex.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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6-Cyano-7-nitroquinoxaline-2,3-dione / pharmacology
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Animals
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Animals, Newborn
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Bicuculline / pharmacology
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Calcium / metabolism*
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Calcium Signaling* / drug effects
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Cerebral Cortex / cytology
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Cerebral Cortex / drug effects
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Cerebral Cortex / growth & development*
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Cerebral Cortex / metabolism*
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Electric Conductivity
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Excitatory Amino Acid Antagonists / pharmacology
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GABA Antagonists / pharmacology
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Hippocampus / cytology
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Hippocampus / drug effects
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Hippocampus / growth & development
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Hippocampus / metabolism
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In Vitro Techniques
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Membrane Potentials / drug effects
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Nerve Net / drug effects
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Nerve Net / metabolism
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Neurons / drug effects
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Neurons / metabolism
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Photons
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Rats
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Rats, Wistar
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Receptors, AMPA / physiology
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Receptors, GABA / physiology
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Receptors, N-Methyl-D-Aspartate / physiology
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Tetrodotoxin / pharmacology
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gamma-Aminobutyric Acid / pharmacology
Substances
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Excitatory Amino Acid Antagonists
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GABA Antagonists
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Receptors, AMPA
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Receptors, GABA
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Receptors, N-Methyl-D-Aspartate
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Tetrodotoxin
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gamma-Aminobutyric Acid
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6-Cyano-7-nitroquinoxaline-2,3-dione
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Calcium
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Bicuculline