Glycine triggers an intracellular calcium influx in oligodendrocyte progenitor cells which is mediated by the activation of both the ionotropic glycine receptor and Na+-dependent transporters

S Belachew; B Malgrange; J M Rigo; B Rogister; P Leprince; G Hans; L Nguyen; G Moonen

doi:10.1046/j.1460-9568.2000.00085.x

Glycine triggers an intracellular calcium influx in oligodendrocyte progenitor cells which is mediated by the activation of both the ionotropic glycine receptor and Na+-dependent transporters

Eur J Neurosci. 2000 Jun;12(6):1924-30. doi: 10.1046/j.1460-9568.2000.00085.x.

Authors

S Belachew¹, B Malgrange, J M Rigo, B Rogister, P Leprince, G Hans, L Nguyen, G Moonen

Affiliation

¹ Department of Human Physiology and Pathophysiology, University of Liège, 17 Place Delcour, B-4020 Liège, Belgium. sbelachew@ulg.ac.be

PMID: 10886333
DOI: 10.1046/j.1460-9568.2000.00085.x

Abstract

Using fluo-3 calcium imaging, we demonstrate that glycine induces an increase in intracellular calcium concentration ([Ca2+]i) in cortical oligodendrocyte progenitor (OP) cells. This effect results from a calcium entry through voltage-gated calcium channels (VGCC), as it is observed only in OP cells expressing such channels, and it is abolished either by removal of calcium from the extracellular medium or by application of an L-type VGCC blocker. Glycine-triggered Ca2+ influx in OP cells actually results from an initial depolarization that is the consequence of the activation of both the ionotropic glycine receptor (GlyR) and Na+-dependent transporters, most probably the glycine transporters 1 (GLYT1) and/or 2 (GLYT2) which are colocalized in these cells. Through this GlyR- and transporter-mediated effect on OP intrcellular calcium concentration [Ca2+]i, glycine released by neurons may, as well as other neurotransmitters, serve as a signal between neurons and OP during development.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Transport Systems, Neutral*
Animals
Bicuculline / pharmacology
Biological Transport / drug effects
Biological Transport / physiology
Calcium / metabolism*
Calcium Channel Blockers / pharmacology
Calcium Channels / metabolism*
Carrier Proteins / metabolism*
Cell Communication / physiology
GABA Antagonists / pharmacology
Glycine / pharmacology*
Glycine Agents / pharmacology
Glycine Plasma Membrane Transport Proteins
Neuroblastoma
Neurons / cytology
Neurons / metabolism
Nifedipine / pharmacology
Oligodendroglia / cytology
Oligodendroglia / metabolism*
Rats
Sodium / metabolism
Stem Cells / cytology
Stem Cells / metabolism*
Strychnine / pharmacology
Tumor Cells, Cultured

Substances

Amino Acid Transport Systems, Neutral
Calcium Channel Blockers
Calcium Channels
Carrier Proteins
GABA Antagonists
Glycine Agents
Glycine Plasma Membrane Transport Proteins
Slc6a5 protein, rat
Slc6a9 protein, rat
Sodium
Strychnine
Nifedipine
Calcium
Glycine
Bicuculline