Abstract
Proliferation and apoptosis are two dynamic, interrelated processes that are regulated by growth factors and cytokines. We investigated the effects of tumor necrosis factor-alpha (TNF-alpha) and transforming growth factor-beta (TGF-beta) on apoptosis and regulation of cell cycle proteins in OLG lineage cells. We found that: (1) both cytokines enhanced apoptosis in neonatal pre-OLGs but only TNFalpha-mediated apoptosis persisted in the presence of a mitogen, fibroblast growth factor (FGF); (2) cell cycle proteins such as p21(waf1/cip1), p27(kip1), cyclin D1 and PCNA were differentially regulated by TNF-alpha and TGF-beta. We conclude that differential modulation of cell cycle proteins by TNF-alpha and TGF-beta contributes to the diversity of their biological effects in OLG lineage cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aging
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Animals
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Apoptosis / drug effects
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CDC2-CDC28 Kinases*
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Cell Cycle Proteins / metabolism*
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Cell Lineage
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Cells, Cultured
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Cyclin D1 / metabolism
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Cyclin-Dependent Kinase 2
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Cyclin-Dependent Kinase Inhibitor p21
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Cyclin-Dependent Kinase Inhibitor p27
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Cyclin-Dependent Kinases / metabolism
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Cyclins / metabolism
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DNA / biosynthesis
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DNA / genetics
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Fibroblast Growth Factors / pharmacology
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In Situ Nick-End Labeling
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Lymphotoxin-alpha / pharmacology*
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Microtubule-Associated Proteins / metabolism
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Mitogens / pharmacology
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Oligodendroglia / cytology
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Oligodendroglia / drug effects*
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Oligodendroglia / metabolism*
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Proliferating Cell Nuclear Antigen / metabolism
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Protein Serine-Threonine Kinases / metabolism
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Rats
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Rats, Sprague-Dawley
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Transforming Growth Factor alpha / pharmacology*
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Tumor Suppressor Proteins*
Substances
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Cdkn1a protein, rat
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Cdkn1b protein, rat
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Cell Cycle Proteins
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Cyclin-Dependent Kinase Inhibitor p21
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Cyclins
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Lymphotoxin-alpha
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Microtubule-Associated Proteins
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Mitogens
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Proliferating Cell Nuclear Antigen
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Transforming Growth Factor alpha
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Tumor Suppressor Proteins
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Cyclin D1
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Cyclin-Dependent Kinase Inhibitor p27
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Fibroblast Growth Factors
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DNA
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Protein Serine-Threonine Kinases
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CDC2-CDC28 Kinases
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Cdk2 protein, rat
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Cyclin-Dependent Kinase 2
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Cyclin-Dependent Kinases